Anti-RAB13 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P51153

Core Product Specifications and Parameters

Biological Background: RAB13 Function and Localization

• RAB13 is a small GTPase of the Rab family, also referred to as Cell growth-inhibiting gene 4 protein (GIG4), and it functions as a principal regulator of intracellular membrane trafficking.
• The protein cycles between inactive GDP-bound and active GTP-bound states to recruit effectors that coordinate vesicle formation, movement, tethering, and fusion.
• RAB13 specializes in endocytic recycling and directs plasma membrane delivery of transmembrane proteins such as occludin (OCLN), thereby governing tight junction assembly and function.
• Beyond occludin transport, RAB13 activates PKA signaling and reorganizes the actin cytoskeleton through effectors PRKACA and MICALL2, respectively, to further modulate tight junction dynamics; it also contributes to the establishment of the Sertoli cell barrier.
• Angiogenesis (via endothelial chemotaxis), neurite outgrowth, and post-Golgi trafficking from the trans-Golgi network to recycling endosomes represent additional RAB13-dependent processes.
• Insulin-stimulated translocation of the glucose transporter GLUT4 to the plasma membrane involves RAB13, implicating it in glucose homeostasis.
• Subcellularly, RAB13 localizes to the cell membrane, cytoplasmic vesicle membranes, tight junctions, Golgi apparatus, trans-Golgi network, recycling endosomes, and lamellipodia.
• High expression is observed in epithelial cells of the intestine, kidney, liver, and in endothelial cells, consistent with its barrier and trafficking functions.

Experimental Guidance and Technical Tips

• The immunogen covers full-length human RAB13 (residues 1–203), supporting recognition of the endogenous protein across applications.
• For Western blot, an expected band around 23 kDa should be observed; verify in a relevant cell or tissue lysate.
• In immunofluorescence/ICC, the antibody may label tight junctions and cytoplasmic vesicles; co-staining with junctional markers is advised.
• ELISA detection may be optimized by using the recombinant immunogen as a positive control.
• Validation in the specific experimental system is recommended, as performance can vary with sample processing and antibody concentration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio accelerates diagnostic development by supplying validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for targets like RAB13. Our just-in-time logistics and dedicated technical support help you secure rare, high-quality raw materials without supply gaps, ensuring seamless progression from concept to clinic.