Anti-Rab12 Rabbit Monoclonal Antibody [KO Validated] for WB and ELISA - Q6IQ22

Core Product Specifications and Parameters

Biological Background: Rab12 Function and Localization

• RAB12 is a member of the Ras superfamily of small GTPases, acting as a molecular switch that cycles between inactive GDP-bound and active GTP-bound conformations to regulate intracellular membrane trafficking.
• It plays a role in the transport of proteins from recycling endosomes to lysosomes, facilitating the degradation of cargo such as the transferrin receptor.
• RAB12 participates in autophagy, localizing to autophagosomal membranes and influencing autophagic processes.
• At the subcellular level, RAB12 is found on membranes of recycling endosomes, lysosomes, the Golgi apparatus, and autophagic vesicles.
• The protein undergoes key post-translational modifications including prenylation for membrane anchoring, as well as phosphorylation and acetylation.
• As a GTPase, RAB12 binds guanine nucleotides and magnesium ions, which are essential for its enzymatic activity.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length human Rab12 protein (aa 1-244), suggesting that the antibody may recognize native Rab12 in Western blot and ELISA applications; validate using appropriate positive and negative controls in your specific sample system.
• For Western blot, consider using lysates from human and mouse cell lines (e.g., HeLa, NIH/3T3) and a knockout control to confirm specificity, given the [KO Validated] designation.
• In ELISA, titrate the antibody against recombinant Rab12 protein to determine optimal working concentrations, and include a standard curve for semi-quantitative analysis.
• Due to antibody bivalency, direct ELISA performance may require pre-coating with recombinant antigen; consider testing indirect formats for better sensitivity.
• Rab12 has a predicted molecular weight of 27 kDa; confirm band migration at the expected weight under reducing conditions, and account for possible post-translational modifications that may cause minor shifts.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our expertise in sourcing rare-target proteins and antibodies streamlines your pipeline from development to commercialization. For projects involving intracellular trafficking or autophagy targets like Rab12, CamelBio provides specialized technical consultation and reliable material supply to accelerate your assay development.