Anti-Rab12 Monoclonal Antibody for IP and ELISA - Q6IQ22

Core Product Specifications and Parameters

Biological Background: Rab-12 Function and Localization

• Ras-related protein Rab-12 is a member of the Rab family of small GTPases, which act as molecular switches in intracellular membrane trafficking.
• Rab-12 cycles between an inactive GDP-bound form and an active GTP-bound form, recruiting downstream effectors to regulate vesicle formation, movement, tethering, and fusion.
• This protein is implicated in the transport of cargo from recycling endosomes to lysosomes, as evidenced by its role in regulating the degradation of the transferrin receptor.
• Rab-12 is also involved in autophagy, a cellular degradation pathway critical for maintaining homeostasis.
• Subcellularly, Rab-12 localizes to recycling endosome membranes, lysosomal membranes, Golgi apparatus membranes, and cytoplasmic vesicles including autophagosomes.
• Post-translational modifications include prenylation (lipoprotein), acetylation, and phosphorylation, which may modulate its membrane association and activity.

Experimental Guidance and Technical Tips

• The immunogen is a full-length recombinant protein (amino acids 1-244), suggesting that this monoclonal antibody recognizes native Rab-12. Validate in the relevant human biological system prior to use.
• For immunoprecipitation (IP), consider using human cell lines that express endogenous or overexpressed Rab-12, and test under both native and denaturing conditions.
• For ELISA, evaluate the antibody’s performance in direct and sandwich formats, and optimize coating and detection conditions for your specific assay.
• As applications are limited to IP and ELISA, perform additional orthogonal validation if required for other techniques.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials, including this anti-Rab12 monoclonal antibody. With validated antibody pairs, optimized monoclonals, and bulk ancillary reagents, we support research in intracellular trafficking and autophagy pathways. Our team also offers customized sourcing for rare targets, ensuring a seamless supply chain from concept to clinic.