Anti-RAB11FIP5 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9BXF6

Core Product Specifications and Parameters

Biological Background: RAB11FIP5 Function and Localization

• Official full name: Rab11 family-interacting protein 5. Also known as Gamma-SNAP-associated factor 1 (Gaf-1), phosphoprotein pp75, and Rab11-interacting protein Rip11. Encoded by the RAB11FIP5 gene (aliases: GAF1, KIAA0857, RIP11) in humans.
• Functions as a Rab11 effector critically involved in protein trafficking from apical recycling endosomes to the apical plasma membrane, a key step in polarized secretion.
• Plays a role in insulin granule exocytosis, linking the protein to regulated secretion pathways in pancreatic beta cells.
• May regulate intracellular transport of V-ATPase in response to extracellular acidosis, suggesting a role in pH homeostasis.
• Subcellular localization is dynamic: detected at recycling endosome membrane, early endosome membrane, Golgi apparatus membrane, secretory vesicle membrane, and mitochondrion membrane, consistent with its role in membrane trafficking.
• Classified as a phosphoprotein; phosphorylation may modulate its function in vesicle transport.
• Tissue expression is broad but low – detected in heart, brain, placenta, lung, liver, adipocytes, kidney, spleen, skeletal muscle, and pancreas.
• Contains a host-virus interaction keyword in UniProt, implying a potential role in cellular response to viral infection.

Experimental Guidance and Technical Tips

• The immunogen was designed against amino acids 354–653 of human RAB11FIP5 (C-terminal region), which is conserved across human, mouse, and rat, supporting the reported cross-reactivity.
• For Western blot, expect a band near 70 kDa. Use appropriate positive controls from human, mouse, or rat cell lines known to express RAB11FIP5. Validate in your specific sample system.
• In immunofluorescence/immunocytochemistry, the antibody is predicted to label endosomal compartments, Golgi, and possibly mitochondria. Co-staining with compartment-specific markers (e.g., EEA1 for early endosomes, TGN46 for Golgi) is recommended to confirm localization.
• ELISA applications may be considered; optimize coating antigen and antibody dilution for best signal-to-noise ratio.
• As a polyclonal antibody, lot-to-lot consistency can vary. It is advisable to titrate the antibody and include appropriate controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a streamlined gateway to high-quality IVD raw materials, supporting projects from early R&D to clinical application. This anti-RAB11FIP5 polyclonal antibody is suited for investigating apical recycling endosome dynamics, insulin secretion, and related membrane trafficking pathways. Beyond single antibodies, we provide validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare or difficult-to-find targets, backed by a global supply network.