Anti-RAB11FIP1 Polyclonal Antibody for WB, ELISA - Q6WKZ4

Core Product Specifications and Parameters

Biological Background: RAB11FIP1 Function and Localization

• RAB11FIP1 (Rab11 family-interacting protein 1), also designated Rab-coupling protein (RCP), is encoded by the RAB11FIP1 gene and undergoes alternative splicing to produce multiple isoforms.
• It functions as a Rab11 effector protein critically involved in the endosomal recycling process, facilitating the return of internalized receptors and lipids to the plasma membrane. Related references: PMID:26032412
• RAB11FIP1 participates in membrane trafficking along the phagocytic pathway and directly contributes to phagocytosis, linking endosomal dynamics to innate immune responses.
• Interaction with RAB14 has been implicated in the process of neurite formation, suggesting roles in neuronal development and cytoskeletal reorganization.
• The protein localizes predominantly to recycling endosomes and is also found on cytoplasmic vesicles and the phagosome membrane, consistent with its trafficking functions.
• Isoform 2 shows tissue-specific expression in brain, heart, testis, lung, spleen, ovary, and small intestine, indicating potential specialized functions in these organs.
• Designated as a phosphoprotein, RAB11FIP1 is subject to post-translational modifications that may regulate its interactions and subcellular distribution.

Experimental Guidance and Technical Tips

• The immunogen spans the C-terminal region (aa 330–540), which may enable selective detection of full-length RAB11FIP1 by Western blot (WB) and ELISA.
• For WB, consider using lysates from human cell lines such as HeLa or HEK293, where endogenous RAB11FIP1 is expressed. The predicted molecular weight is ~137 kDa, but observed bands may shift due to phosphorylation or other modifications; validate in the relevant sample system.
• Ensure proper loading controls and consider membrane fraction enrichment if signals are weak, given the protein’s endosomal/vesicular localization.
• This polyclonal antibody is suitable for ELISA-based assays; optimize coating and detection conditions against recombinant or native RAB11FIP1 as appropriate.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutions a streamlined supply channel for high-quality IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies like this anti-RAB11FIP1 product for endosomal trafficking and phagocytosis research. Our portfolio spans bulk ancillary reagents and rare target sourcing, complemented by technical consulting to accelerate your assay development from concept to clinic. We serve as a reliable one-stop partner, ensuring consistent access to critical raw materials for your diagnostic and life science workflows.