Anti-RAB11B Polyclonal Antibody for WB, IF/ICC, ELISA - Q15907

Core Product Specifications and Parameters

Biological Background: RAB11B Function and Localization

• Ras-related protein Rab-11B (RAB11B), also known as GTP-binding protein YPT3, is a member of the Ras superfamily of small GTPases. It cycles between GDP-bound inactive and GTP-bound active states, functioning as a molecular switch in intracellular membrane trafficking.
  Related references: PMID:14627637, PMID:19029296, PMID:19244346

• RAB11B is a key regulator of endocytic recycling, controlling the apical recycling of transmembrane proteins such as CFTR, ENaC, potassium channels, and voltage-dependent L-type calcium channels. It also governs constitutive and regulated secretory pathways, including insulin granule exocytosis.
  Related references: PMID:14627637, PMID:19029296, PMID:19244346

• The protein is essential for melanosome transport and release from melanocytes, and it modulates V-ATPase intracellular transport in response to extracellular acidosis.
  Related references: PMID:14627637, PMID:19029296, PMID:19244346

• RAB11B promotes ciliogenesis by forming a ciliary targeting complex with Rabin8/RAB3IP, ASAP1, RAB11FIP3, and ARF4, facilitating preciliary vesicular trafficking to the mother centriole. Conversely, LPAR1 signaling-mediated phosphorylation of WDR44 disrupts this complex, inhibiting cilia growth.
  Related references: PMID:25673879, PMID:31204173

• Additionally, RAB11B interacts with RABL3 to drive ciliary vesicle formation, highlighting its multifaceted roles in primary cilium assembly.
  Related references: PMID:36052645

• Subcellularly, RAB11B is predominantly associated with recycling endosome membranes, but it is also found on cytoplasmic vesicles, secretory vesicles, synaptic vesicle membranes, and phagosome membranes, reflecting its dynamic trafficking functions.

• Post-translational modifications, including acetylation, citrullination, methylation, and prenylation, regulate its activity, localization, and protein interactions. Alternative splicing generates multiple isoforms, and mutations are linked to intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 100–200 of human RAB11B, which lies within a region likely conserved across species, consistent with reported cross-reactivity to mouse and rat. This peptide-based design may recognize both denatured and native protein conformations.
• For Western blotting, a band at approximately 24 kDa is expected. Given that RAB11B undergoes prenylation and other modifications, minor size shifts or additional bands may be observed in some samples; validate with positive controls such as HeLa or HEK293 cell lysates.
• In immunofluorescence/immunocytochemistry, permeabilization is typically required for intracellular targets. Endosomal and vesicular staining patterns should be expected, with possible enrichment at recycling endosomes.
• For ELISA applications, the peptide immunogen can serve as a coating antigen, but careful optimization of antibody dilution and blocking conditions is advised.
• As with any polyclonal antibody, lot-to-lot consistency should be verified using a standardized positive control. Consider validating in the relevant sample system (human, mouse, or rat) to confirm performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes one-stop access to a comprehensive portfolio of IVD raw materials, including validated antibodies like this anti-RAB11B polyclonal antibody for membrane trafficking studies. Our supply capabilities extend to optimized monoclonal/polyclonal antibody pairs, bulk ancillary reagents, and assistance with rare-target raw-material sourcing, all designed to streamline your assay development from concept to clinic. Let CamelBio be your sourcing bridge to accelerate innovation in endocytic recycling and ciliogenesis research.