Anti-RAB11A/RAB11B Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P62491 / Q15907

Core Product Specifications and Parameters

Biological Background: RAB11A and RAB11B Function and Localization

• RAB11A (also known as YL8) is a small GTPase that cycles between inactive GDP-bound and active GTP-bound forms, acting as a master regulator of intracellular membrane trafficking. Related references: PMID:15601896, PMID:15689490, PMID:17462998
• RAB11A plays a critical role in endocytic recycling and forms a Rab11/RAB11FIP3/dynein complex that moves peripheral sorting endosomes along microtubules to generate the endosomal recycling compartment (ERC). Related references: PMID:20026645
• It is a major regulator of membrane delivery during cytokinesis and, together with MYO5B and RAB8A, participates in epithelial cell polarization. Related references: PMID:15601896, PMID:21282656
• RAB11A, in complex with Rabin8/RAB3IP and other factors, promotes apical membrane initiation and lumenogenesis via transcytosis of PODXL. Related references: PMID:20890297
• It facilitates the trafficking of CFTR to the plasma membrane and transferrin recycling, and is required for NPC1L1 transport. Related references: PMID:17462998, PMID:19542231
• RAB11A contributes to ciliogenesis initiation by forming a ciliary targeting complex that includes ASAP1, Rabin8/RAB3IP, and ARF4, and its interaction with WDR44 is regulated by LPAR1 signaling. Related references: PMID:25673879, PMID:31204173
• It participates in the export of neosynthesized proteins through a Rab8-Rab10-Rab11-endosomal route via interaction with WDR44 and promotes ciliary vesicle formation with RABL3. Related references: PMID:32344433, PMID:36052645
• Subcellular localization includes the cell membrane, endosome membrane, recycling endosome membrane, cleavage furrow, phagosome membrane, Golgi apparatus, trans-Golgi network, cytoplasmic vesicles, and cilium. Post-translational modifications include acetylation, prenylation, and methylation.
• RAB11B is a closely related Rab GTPase that shares high sequence homology with RAB11A and likely participates in similar endosomal trafficking events.

Experimental Guidance and Technical Tips

• For Western blot, the predicted molecular weight of RAB11A is ~24 kDa; however, prenylation and membrane association may cause slight shifts. Validate in your system.
• For immunohistochemistry and immunofluorescence, fixation and permeabilization conditions may affect antigen accessibility; optimize protocols for your tissue/cell type.
• The immunogen peptide corresponds to a conserved region between RAB11A and RAB11B, so the antibody may recognize both proteins; confirm specificity through knockdown or overexpression experiments.
• As a polyclonal antibody, lot-to-lot consistency should be monitored if used in quantitative assays.
• ELISA can be used for quantitation, but ensure proper coating and blocking to reduce background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-RAB11A/RAB11B polyclonal antibody is part of our extensive portfolio of membrane trafficking and oncology research reagents. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your assay development.