Anti-RAB11A Polyclonal Antibody for WB, IF/ICC, ELISA - P62491

Core Product Specifications and Parameters

Biological Background: RAB11A Function and Localization

• Protein Identity: RAB11A, also known as YL8, is a member of the Ras-related Rab family of small GTPases.
• GTPase Cycle and Trafficking Control: RAB11A cycles between an inactive GDP-bound state and an active GTP-bound state, enabling it to recruit downstream effectors responsible for vesicle formation, movement, tethering, and fusion, thereby functioning as a key regulator of intracellular membrane trafficking.
  Related references: PMID:15601896, PMID:15689490, PMID:17462998
• Endocytic Recycling: RAB11A regulates endocytic recycling and forms a functional complex with RAB11FIP3 and dynein to transport peripheral sorting endosomes along microtubules to the centrosome, generating the endosomal recycling compartment (ERC).
  Related references: PMID:20026645
• Cytokinesis: It acts as a major regulator of membrane delivery during cytokinesis.
  Related references: PMID:15601896
• Cell Polarization and Lumenogenesis: Together with MYO5B, RAB8A, the exocyst complex, and other partners, RAB11A promotes epithelial cell polarization, transcytosis of PODXL, apical surface formation, and lumenogenesis.
  Related references: PMID:21282656, PMID:20890297
• Protein Trafficking: RAB11A participates in the trafficking of various cargoes, including CFTR to the plasma membrane, transferrin recycling, NPC1L1 transport, and CDH1 sorting and basolateral transport.
  Related references: PMID:17462998, PMID:19542231, PMID:15689490
• Ciliogenesis: The protein regulates ciliary vesicle formation and cilia growth initiation by forming a ciliary targeting complex with Rabin8/RAB3IP, RAB11FIP3, ARF4, and ASAP1, and by interacting with RABL3. This process is inhibited upon LPAR1 signaling via phosphorylated WDR44.
  Related references: PMID:25673879, PMID:31204173, PMID:36052645
• Subcellular Localization: RAB11A is found on multiple membrane compartments, including the cell membrane, endosome membrane, recycling endosome membrane, cleavage furrow, phagosome membrane, Golgi apparatus membrane, trans-Golgi network, cytoplasmic vesicle membrane, and primary cilium.
• Post-Translational Modifications and Keywords: UniProt lists RAB11A with diverse annotations: it is subject to acetylation and methylation, is prenylated (lipid-anchored), and participates in transport, cell cycle, and ciliary processes.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an internal sequence (a.a. 100–200) of human RAB11A; this region is likely to be accessible in both native and denatured protein forms, supporting use in WB and ELISA. For IF/ICC, consider fixation and permeabilization protocols that preserve the epitope while allowing antibody access.
• Endogenous RAB11A expression is widespread, but signal intensity may vary among cell lines and tissues; include appropriate positive and negative controls for each application.
• Cross-reactivity with rat has been reported; homology between human and rat RAB11A in the immunogen region supports this. For other species, verify sequence alignment before use.
• For Western blot, a band around 24 kDa is expected; use a standard molecular weight marker and validate the band in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic and research laboratories with comprehensive access to IVD raw materials, technical services, and consulting. This anti-RAB11A polyclonal antibody supports researchers in membrane trafficking and intracellular transport studies, and is part of our broad portfolio of validated antibodies and ancillary reagents. For larger-scale needs, custom development, or rare-target sourcing, contact us to streamline your procurement from concept to clinic.