Anti-RAB10 Rabbit Polyclonal Antibody for WB and ELISA - P61026

Core Product Specifications and Parameters

Biological Background: RAB10 Function and Localization

• RAB10 encodes the small GTPase Rab-10, a key regulator of intracellular membrane trafficking.
• It cycles between GDP- and GTP-bound states to control vesicle formation, movement, tethering, and fusion. Related references: PMID:21248164
• Primarily mediates biosynthetic transport from the Golgi to the plasma membrane, crucial for processes like GLUT4 translocation and TLR4 surface delivery (PubMed:21248164; By similarity). Related references: PMID:21248164
• Facilitates asymmetric protein transport to the plasma membrane and contributes to axonogenesis and basolateral trafficking in epithelial cells (PubMed:16641372). Related references: PMID:16641372
• Along with LRRK2, RAB8A, and RILPL1, regulates ciliogenesis; LRRK2-mediated phosphorylation at Thr-73 inhibits cilia formation (PubMed:30398148). Related references: PMID:30398148
• Participates in a Rab8-Rab10-Rab11-dependent endosomal export route for specific newly synthesized proteins (PubMed:32344433). Under lysosomal stress, it promotes extracellular release of lysosomal content via EHBP1/EHNP1L1 (PubMed:30209220). Related references: PMID:32344433, PMID:30209220
• Localizes to the Golgi, TGN, endosomes, recycling endosomes, cytoplasmic vesicles, phagosomes, ER, cilium basal body, and lysosomes. Expressed in the hippocampus, neutrophils, and testis.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 98–198, a central region, therefore the antibody may recognize both GDP- and GTP-bound RAB10; for functional studies, consider combining with GTPase activity assays.
• For WB, the predicted molecular weight is ~23 kDa; use a 12–15% gel for clear separation and validate in relevant cell lines (e.g., HeLa, HEK293) expressing endogenous RAB10.
• In ELISA, optimize antigen coating concentration and antibody dilution; pre‑adsorption against the immunogen can help confirm binding specificity.
• Given the mouse cross‑reactivity, the antibody is suitable for mouse tissue or cell studies; validation in knockout controls is recommended to confirm specificity.
• Due to RAB10’s dynamic membrane association, subcellular fractionation to enrich membrane fractions may enhance detection signal.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For RAB10-focused studies in intracellular trafficking, ciliogenesis, or neurodegenerative diseases, CamelBio supplies validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom rare-target raw-material sourcing to accelerate your assay development and production.