Anti-R9AP/RGS9BP Polyclonal Antibody for WB and ELISA - Q6ZS82

Core Product Specifications and Parameters

Biological Background: R9AP/RGS9BP Function and Localization

• R9AP/RGS9BP (Regulator of G protein signaling 9-binding protein), also known as RGS9-anchoring protein, is a membrane protein encoded by the RGS9BP gene.
• It acts as a key regulator of G protein-coupled receptor (GPCR) signaling in phototransduction, specifically in the recovery phase of visual transduction.
• R9AP serves as a membrane anchor that targets the RGS9-1 isoform to the photoreceptor outer segment, the site of phototransduction.
• By interacting with RGS9-1, it enhances the GTPase-accelerating activity, allowing rapid termination of the visual signal.
• It also controls the proteolytic stability of RGS9-1, likely protecting it from degradation.
• Subcellularly localized to the membrane, with features including a coiled coil domain and a transmembrane helix (as per UniProt keywords).
• Keywords from UniProt include Sensory transduction, Signal transduction inhibitor, Vision, and Membrane, underscoring its role in visual signaling.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-107), which may detect both full-length and truncated forms. Validate specificity in your sample system.
• For Western blot, a band near 25 kDa is anticipated in human, mouse, and rat retina lysates; consider using appropriate tissue controls.
• In ELISA, coating with the recombinant immunogen or native protein is recommended; optimize coating concentration and detection conditions.
• Always include positive and negative controls to confirm reactivity and minimize nonspecific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering a comprehensive portfolio including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This R9AP/RGS9BP antibody supports research in vision and GPCR signaling, and our team can assist with rare target raw-material sourcing and technical consultation to accelerate your project from concept to clinic.