Anti-QSOX1 Polyclonal Antibody for WB, ELISA - O00391

Core Product Specifications and Parameters

Biological Background: QSOX1 Function and Localization

• QSOX1, also known as Sulfhydryl oxidase 1 or Quiescin Q6, is an FAD-dependent enzyme that catalyzes the oxidation of sulfhydryl groups to disulfides, reducing oxygen to hydrogen peroxide.
  Related references: PMID:17331072, PMID:18393449, PMID:23704371
• It facilitates disulfide bond formation in extracellular proteins, including laminin, and is critical for normal cell-cell adhesion and fibroblast migration.
  Related references: PMID:22801504, PMID:23867277, PMID:30367560
• The protein localizes to the Golgi apparatus membrane and is also secreted into the extracellular milieu.
• QSOX1 is broadly expressed, with high levels in heart, placenta, lung, liver, skeletal muscle, and pancreas, and low levels in brain and kidney.
• As a member of the FAD-dependent oxidoreductase family, it contains a flavin adenine dinucleotide (FAD) cofactor required for catalytic activity.
• Post-translational modifications include glycosylation and phosphorylation, and the protein features an N-terminal signal peptide and a transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-330) of human QSOX1; for Western blot, expect a band around 83 kDa, but note that alternative splicing may produce additional isoforms.
• This polyclonal antibody batch is supplied as a polyclonal antibody from rabbit; consider titration and positive/negative controls in your specific sample type.
• Cross-reactivity is reported for human and mouse; for other species, validate performance independently.
• While validated for WB and ELISA, suitability for other applications (e.g., IHC, IF) has not been assessed and should be determined experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for diagnostic manufacturers and research groups, offering one-stop access to validated antibodies, bulk reagents, and technical support. The QSOX1 polyclonal antibody serves as a robust tool for investigating oxidative protein folding and extracellular matrix biology, with our supply network ensuring reliable, scalable sourcing for long-term assay development. For rare targets or customization needs, our team provides rapid sourcing and consultation to keep your projects on track.