Anti-QPRT Polyclonal Antibody for WB and ELISA - Q15274

Core Product Specifications and Parameters

Biological Background: QPRT Function and Localization

• QPRT encodes nicotinate-nucleotide pyrophosphorylase [carboxylating], also called quinolinate phosphoribosyltransferase (QPRTase/QAPRTase), a key enzyme in the pyridine nucleotide biosynthesis pathway.
• The enzyme catalyzes the conversion of quinolinic acid (QA) to nicotinic acid mononucleotide, playing a critical role in the catabolism of quinolinic acid and de novo NAD+ synthesis.
• QPRT belongs to the glycosyltransferase family and functions as a transferase, transferring phosphoribosyl groups in the biosynthesis of nicotinate nucleotides.
• Structural studies (3D-structure keyword) have been performed, and the protein has been identified in proteomics analyses.
• The molecular weight is approximately 31 kDa (calculated 30.8 kDa), and it is conserved across human, mouse, and rat species, enabling cross-reactivity in research.

Experimental Guidance and Technical Tips

• The immunogen is a full-length recombinant QPRT protein (aa 1-297), which likely generates antibodies recognizing multiple epitopes. For WB, consider using whole cell lysates or tissue extracts expressing QPRT, and validate with appropriate positive and negative controls.
• For ELISA, the polyclonal antibody can be used as a capture or detection reagent; optimal dilutions must be empirically determined in the specific assay format.
• Cross-reactivity with mouse and rat is expected, but sequence alignment should be verified to confirm epitope conservation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with a comprehensive portfolio of IVD raw materials, including validated antibodies like this anti-QPRT polyclonal antibody for quinolinic acid metabolism and NAD+ biosynthesis studies. As a one-stop partner, CamelBio offers optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and customized sourcing solutions to accelerate your assay development from concept to clinic.