Anti-QKI Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - Q96PU8

Core Product Specifications and Parameters

Biological Background: QKI Function and Localization

• QKI (protein quaking) is a KH domain-containing RNA-binding protein. It has multiple isoforms that localize to the nucleus, cytoplasm, and stress granules, enabling diverse regulatory roles.
• As an RNA reader, QKI binds specific RNAs to regulate pre-mRNA splicing, circular RNA formation, mRNA export, stability, and translation. It recognizes the 5'-NACUAAY-N(1,20)-UAAY-3' core sequence. Related references: PMID:22398723, PMID:23630077, PMID:25768908
• QKI plays a central role in myelinization by stabilizing myelin-related mRNAs (e.g., MBP, SIRT2), regulating mRNA transport, and indirectly controlling MAG alternative splicing. Related references: PMID:16641098
• QKI is involved in mRNA storage into stress granules, apoptosis, lipid deposition, interferon response, and glial cell development. Related references: PMID:25768908, PMID:31829086, PMID:34428287
• A cytosolic isoform recognizes N7-methylguanine (m7G) modified mRNAs; interaction with G3BP1 localizes these mRNAs to stress granules, suppressing translation. Related references: PMID:37379838
• QKI promotes monocyte differentiation via pre-mRNA splicing regulation, and delays macrophage differentiation by promoting CSF1R mRNA degradation. Related references: PMID:27029405, PMID:22398723
• QKI is expressed in the frontal cortex and is down-regulated in the brain of schizophrenic patients.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 242-341 of human QKI; consider this epitope when designing experiments or selecting detection modalities.
• For WB, a band around 38 kDa is expected for human QKI; validate in the relevant sample system and optimize loading controls.
• IHC-P and IF/ICC may require optimization of antigen retrieval methods and permeabilization conditions for your specific tissues or cells.
• The antibody cross-reacts with mouse and rat QKI, enabling multi-species studies; verify cross-reactivity in your model system as needed.
• For IP, consider using appropriate isotype controls and confirm specificity with downstream applications.
• ELISA can be employed for quantification; ensure the peptide epitope is accessible under your assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to high-quality IVD raw materials for targets such as QKI, essential for RNA metabolism and myelinization studies. Our portfolio includes validated antibody pairs, optimized monoclonal antibodies, and ancillary reagents to support your assay development and manufacturing needs. Leverage our global sourcing network to secure rare target raw materials and accelerate your project from concept to clinic.