Anti-QKI Monoclonal Antibody for WB - Q96PU8

Core Product Specifications and Parameters

Biological Background: QKI Function and Localization

• QKI functions as an RNA reader protein that regulates key RNA metabolic processes, including pre-mRNA splicing, circular RNA (circRNA) biogenesis, mRNA export, stability, and translation (PubMed:22398723, PubMed:23630077, PubMed:25768908). Related references: PMID:22398723 PMID:23630077 PMID:25768908

• Recognizes a specific 5'-NACUAAY-N(1,20)-UAAY-3' RNA core motif and also serves as an m7G reader, binding internal N7-methylguanine-modified transcripts (PubMed:23630077, PubMed:37379838). Related references: PMID:23630077 PMID:37379838

• Plays a central role in oligodendrocyte differentiation and myelinization by stabilizing mRNAs such as MBP, SIRT2, and CDKN1B, and regulating alternative splicing of MAG pre-mRNA.

• Nuclear isoforms negatively regulate the interferon response by binding MAVS and FN1 pre-mRNAs, repressing their expression (PubMed:31829086, PubMed:34428287). Related references: PMID:31829086 PMID:34428287

• Cytosolic isoforms mediate stress granule localization of m7G mRNAs via G3BP1 interaction, suppressing translation, and also regulate angiogenesis and apoptosis (PubMed:37379838, PubMed:32732889). Related references: PMID:37379838 PMID:32732889

• Involved in muscle development and cardiomyocyte contractile function through alternative splicing regulation, and associated with smooth muscle development (PubMed:31331967). Related references: PMID:31331967

• Highly expressed in the frontal cortex; down-regulation observed in the brain of schizophrenic patients.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein encompassing amino acids 1-341 of human QKI, covering the majority of the protein including its KH RNA-binding domains. This broad coverage may ensure detection across multiple isoforms and functional states.
• For Western blot (WB), the predicted molecular weight is approximately 38 kDa; however, due to alternative splicing and post-translational modifications, multiple bands or a range may be observed. Validation using knockdown or knockout controls is recommended.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), antibody penetration and epitope accessibility may require optimized antigen retrieval and permeabilization protocols. Nuclear and cytoplasmic localization should be confirmed with appropriate subcellular markers.
• For ELISA applications, consider using recombinant human QKI protein as a standard and validate the assay with known positive and negative samples to establish dynamic range and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with seamless access to high-quality IVD raw materials, technical services, and consulting. This QKI monoclonal antibody supports neuroscience and RNA biology research, and CamelBio can further supply validated antibody pairs, bulk ancillary reagents, or assist in sourcing rare target raw materials for your development pipeline.