Anti-QARS Rabbit Polyclonal Antibody for WB, IHC, IF, ELISA - P47897

Core Product Specifications and Parameters

Biological Background: QARS Function and Localization

• Glutamine--tRNA ligase (QARS), also known as glutaminyl-tRNA synthetase (GlnRS), is encoded by the QARS1 gene. Alternative splicing generates multiple isoforms.
• Catalyzes the ligation of glutamine to its cognate tRNA, an essential step in protein biosynthesis.
  Related references:
  PMID:26869582
• Plays a critical role in brain development; loss-of-function mutations are associated with primary microcephaly and epileptic encephalopathy.
  Related references:
  PMID:24656866
• Predominantly localizes to the cytoplasm and cytosol.
• Highly expressed in the fetal cerebral cortex, particularly in ventricular and subventricular zones.
• Subject to post-translational modifications, including acetylation and phosphorylation, which may modulate its activity.
• Belongs to the class I aminoacyl‑tRNA synthetase family, characterized by conserved ATP‑binding and nucleotide‑binding domains.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N‑terminal 250 amino acids; therefore, the antibody may recognize full‑length QARS and any isoforms retaining this region.
• For Western blotting (WB), a band at ~88 kDa is anticipated. Include appropriate positive (e.g., HEK293, HeLa) and negative controls, and validate against the species of interest (human or mouse).
• Immunohistochemistry (IHC‑P) may require antigen retrieval. Staining is expected in the cytoplasm of QARS‑expressing tissues; fetal brain or tumor sections can serve as positive controls.
• In immunofluorescence (IF/ICC), a cytoplasmic signal should be observed. Optimize fixation and permeabilization conditions.
• For ELISA, use recombinant QARS protein or peptide as the coating antigen. Pair with a suitable detection antibody if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one‑stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For QARS and translation‑focused research, we supply validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. Our sourcing capabilities also extend to rare target raw‑material procurement, ensuring you have the critical components needed for assay development.