Anti-Pyruvate carboxylase (PC) Rabbit Polyclonal Antibody for WB, IF/ICC, IP, ELISA - P11498

Core Product Specifications and Parameters

Biological Background: Pyruvate carboxylase (PC) Function and Localization

• Pyruvate carboxylase (PC) is a mitochondrial enzyme that catalyzes the ATP-dependent carboxylation of pyruvate to oxaloacetate, a critical anaplerotic reaction replenishing the tricarboxylic acid cycle intermediates.
• The reaction occurs in two steps: the biotin prosthetic group is first carboxylated using bicarbonate and ATP, followed by transfer of the carboxyl group to pyruvate to form oxaloacetate.
• PC plays a tissue-specific role in gluconeogenesis in the liver and kidney, and in de novo lipogenesis in adipose tissue, liver, and brain, linking carbohydrate and lipid metabolism.
• Located in the mitochondrial matrix, PC is synthesized as a precursor with a transit peptide that directs it to mitochondria.
• PC is a biotin-dependent, multifunctional ligase that requires manganese or magnesium as a metal cofactor and is regulated by acetylation and phosphorylation.
• Mutations in the PC gene are associated with pyruvate carboxylase deficiency, a rare autosomal recessive metabolic disorder.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 879–1178 of human PC, targeting the C-terminal region; consider evaluating reactivity with endogenous PC in the relevant species and tissue.
• For Western blotting, a band near 130 kDa is expected; include appropriate molecular weight markers and validate with positive controls.
• Optimize antibody dilution for each application (WB, IF/ICC, IP, ELISA) using a titration series and appropriate blocking conditions.
• Cross-reactivity is reported for human, mouse, and rat PC; due to polyclonal nature, lot-to-lot consistency should be verified in the intended assay.

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CamelBio partners with diagnostic manufacturers, laboratories, and research institutes to supply a comprehensive portfolio of IVD raw materials, including validated antibodies for metabolic markers like PC. Our offering extends beyond individual reagents to optimized antibody pairs, bulk ancillary reagents, and customization for rare targets. From concept to clinic, we streamline your sourcing with technical support and supply chain reliability.