Anti-Pyruvate Carboxylase (PC) Rabbit mAb for WB and ELISA - P11498

Core Product Specifications and Parameters

Biological Background: Pyruvate Carboxylase Function and Localization

• Pyruvate carboxylase (PC) is a mitochondrial enzyme that catalyzes the ATP-dependent carboxylation of pyruvate to oxaloacetate, playing a key role in gluconeogenesis and lipid biosynthesis.
• The enzyme undergoes a two-step reaction: first, biotin carboxylation via ATP hydrolysis; second, transfer of the carboxyl group to pyruvate.
• Tissue-specific expression: in liver and kidney, PC initiates glucose synthesis; in adipose tissue, liver, and brain, it contributes to lipid synthesis.
• Encoded by the PC gene, the human protein is a biotin-dependent carboxylase and a member of the multifunctional enzyme family.
• Located in the mitochondrial matrix, PC contains a transit peptide for mitochondrial import.
• Post-translational modifications include acetylation and phosphorylation, and the protein is associated with disease variants.
• Contains binding sites for ATP, biotin, and manganese, essential for its catalytic activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of human PC; this region is conserved in mouse and rat, which aligns with the observed cross-reactivity.
• For Western blot, a band around 130 kDa is expected; consider using a reducing gel and appropriate loading controls due to the large molecular weight.
• In ELISA, the antibody may be used for either direct or capture-detection formats; validate with recombinant PC or tissue lysates where PC is highly expressed (e.g., liver or kidney samples).
• Since PC is a mitochondrial protein, sample preparation may require mitochondrial enrichment protocols for optimal detection.
• Due to the presence of biotin in the target, avoid biotin-containing blocking reagents if using biotin-based detection systems to reduce background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, from validated antibody pairs and monoclonal antibodies to bulk ancillary reagents. This antibody targeting pyruvate carboxylase supports studies in gluconeogenesis and lipid metabolism. For unique research needs, our team also sources rare target raw materials to accelerate your assay development.