Anti-Pyrophosphatase 1 Monoclonal Antibody for WB and ELISA - Q15181

Core Product Specifications and Parameters

Biological Background: Pyrophosphatase 1 (PPA1) Function and Localization

• PPA1 encodes inorganic pyrophosphatase, a ubiquitously expressed enzyme that catalyzes the hydrolysis of inorganic diphosphate into two phosphate molecules. Related references: PMID:33036755, PMID:38428647
• The protein also possesses MAP kinase serine/threonine phosphatase activity toward JNK, and in neuronal cells may negatively regulate neurite growth via JNK dephosphorylation.
• PPA1 is primarily localized in the cytoplasm, consistent with its role in metabolic phosphate hydrolysis.
• Post-translational modifications include acetylation and phosphorylation, and the enzyme requires magnesium ions as cofactors for its hydrolase activity.
• The 3D structure of PPA1 has been solved, and it is classified under the hydrolase family.
• Additional aliases include PP, PP1, IOPPP, and HEL-S-66p, reflecting its identification in various tissues and contexts.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100), which is conserved across human, mouse, and rat; consider this antibody for cross-species studies.
• For Western blotting, the predicted molecular weight is 33 kDa; validate in the relevant sample system with appropriate loading controls.
• For ELISA, the antibody may be used as capture or detection reagent; titrate and optimize conditions for each assay format.
• Because the target has hydrolase and phosphatase functions, ensure sample preparation preserves native conformation if studying functional forms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing bridge for IVD raw materials, offering validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents for pyrophosphatase research. Whether you need high-specificity Pyrophosphatase 1 antibodies or rare-target raw materials for metabolic and signaling studies, our integrated supply chain supports every stage from concept to clinic.