Anti-PYGM Rabbit Polyclonal Antibody for WB, ELISA - P11217

Core Product Specifications and Parameters

Biological Background: PYGM Function and Localization

• PYGM encodes the muscle-specific isoform of glycogen phosphorylase (myophosphorylase), a key enzyme in glycogen breakdown.
• It catalyzes the rate-limiting step of glycogenolysis: the phosphorolytic cleavage of α-1,4-glycosidic linkages in glycogen to release glucose-1-phosphate, thereby maintaining glucose homeostasis.
• The enzyme is subject to allosteric regulation by metabolites such as AMP, ATP, and glucose-6-phosphate, allowing rapid adjustment of glycogenolysis to cellular energy demands.
• PYGM is predominantly expressed in skeletal muscle, and its deficiency causes glycogen storage disease type V (McArdle disease), characterized by exercise intolerance and muscle cramps.
• Post‑translational modifications include phosphorylation (which converts the inactive b form to the active a form) and acetylation, modulating enzyme activity.
• The protein requires pyridoxal phosphate as a cofactor and exists as a homodimer; alternative splicing produces multiple transcript variants.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 743–842) of human PYGM; consider that the antibody may detect full-length protein and potentially some truncated isoforms.
• For Western blotting, the predicted molecular weight is ~97 kDa; validate the band size using appropriate positive controls (e.g., human skeletal muscle lysates).
• Cross‑reactivity with mouse and rat PYGM makes this antibody suitable for comparative studies across species; however, verify reactivity in your specific sample matrix.
• For ELISA applications, optimize coating antigen and antibody dilutions empirically, as quantitative performance can vary with assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing IVD manufacturers and research laboratories with a comprehensive range of high‑quality raw materials, from validated antibodies like this anti‑PYGM polyclonal antibody to ancillary reagents and bulk custom orders. Whether you need optimized antibody pairs, rare‑target sourcing, or end‑to‑end technical consulting, our global supply chain ensures reliable access to critical components for glycogen metabolism and muscle disease research. Partner with us to streamline your raw material procurement from concept to clinical application.