Anti-PYGL Rabbit Polyclonal Antibody - P06737

Core Product Specifications and Parameters

Biological Background: PYGL Function and Localization

• PYGL encodes the liver form of glycogen phosphorylase, a central enzyme in glycogen catabolism.
• It catalyzes the rate-limiting phosphorolytic cleavage of glycogen to release glucose-1-phosphate.
• This reaction is crucial for maintaining blood glucose homeostasis, particularly during fasting.
• The protein is localized to the cytoplasm (cytosol) of hepatocytes.
• PYGL is an allosteric enzyme regulated by metabolites and requires pyridoxal phosphate as a cofactor.
• Post-translational modifications include acetylation and phosphorylation, modulating enzyme activity.
• Mutations in PYGL cause glycogen storage disease type VI (Hers disease), characterized by hepatomegaly and fasting hypoglycemia.
• Alternative splicing generates multiple transcript variants encoding different isoforms.

Experimental Guidance and Technical Tips

• The immunogen covers a C-terminal region (aa 690-847) that may recognize all splice variants if the sequence is conserved; verify with your system.
• As a rabbit polyclonal, it provides high sensitivity; for quantitative assays, we recommend validating lot-to-lot consistency.
• Western blot analysis typically detects a band at ~97 kDa in human, mouse, or rat liver lysates; confirm with appropriate controls.
• For IHC-P, optimization of antigen retrieval may be required; liver tissue sections serve as a positive control.
• The antibody is suitable for immunoprecipitation and ELISA; titrate to determine optimal working concentrations for your samples.
• Cross-reactivity with mouse and rat makes it suitable for rodent models of glycogen metabolism; validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, recombinant proteins, and ancillary reagents. For glycogen metabolism and liver disease research, we provide custom development and bulk supply services to support your assay and kit manufacturing. Our technical team bridges the gap from concept to clinical application, ensuring reliable sourcing for your critical projects.