Anti-PYCRL Polyclonal Antibody for WB and ELISA - Q53H96

Core Product Specifications and Parameters

Biological Background: PYCRL Function and Localization

• PYCRL (Pyrroline-5-carboxylate reductase 3), also known as PYCR3, is an enzyme that catalyzes the final step in proline biosynthesis, responsible for converting pyrroline-5-carboxylate (P5C) into L-proline using NAD(P)H as a cofactor. This reaction is specifically linked to the ornithine-derived proline pathway. Related references: PMID:23024808 PMID:36414121
• The protein is predominantly localized in the cytoplasm, where it acts on cytoplasmic P5C generated from ornithine catabolism.
• PYCRL is a member of the pyrroline-5-carboxylate reductase family, alongside PYCR1 and PYCR2; however, PYCRL is uniquely dedicated to the ornithine-to-proline route, whereas the other isoforms function primarily in glutamate-derived proline synthesis.
• Alternative splicing of the PYCR3 gene produces multiple transcript variants, which may give rise to protein isoforms with altered functions or regulatory properties.
• Post-translational modifications such as acetylation have been reported, potentially modulating enzymatic activity or stability.
• The calculated molecular weight of the PYCRL protein is approximately 28.7 kDa, consistent with the observed band at 29 kDa in western blotting experiments.

Experimental Guidance and Technical Tips

• For western blot (WB) assays, prepare whole-cell lysates from human or mouse cell lines known to express PYCRL; a single band near 29 kDa is expected. Due to polyclonal nature, multiple bands may appear if cross-reactive epitopes are present; consider using peptide blocking to confirm specificity.
• In ELISA applications, this antibody can be employed for direct or sandwich ELISA to quantify PYCRL in biological samples; titrate the antibody to achieve optimal signal-to-noise ratio.
• Cross-reactivity with mouse PYCRL has been demonstrated; for other species, sequence homology analysis can guide potential reactivity, but empirical validation is essential.
• Given the immunogen spans residues 13–286, the antibody likely recognizes multiple linear and conformational epitopes; denaturing and reducing conditions in WB are generally compatible.
• When studying proline metabolism, combine this antibody with inhibitors of ornithine aminotransferase to modulate PYCRL substrate levels and assess pathway flux.
• Always include appropriate controls such as PYCRL knockout cell lines or siRNA-treated samples to confirm band identity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive sourcing solution for IVD manufacturers, laboratories, and research institutes, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. This anti-PYCRL polyclonal antibody supports investigations into proline metabolism, amino-acid biosynthesis, and related pathological conditions. For rare target reagents or custom antibody development needs, CamelBio's technical team can assist in bridging the gap from concept to clinical application.