Anti-PYCR1 Rabbit Monoclonal Antibody for WB, IF/ICC, IF-P, IHC-P, ELISA - P32322

Core Product Specifications and Parameters

Biological Background: PYCR1 Function and Localization

• PYCR1 encodes pyrroline-5-carboxylate reductase 1, a mitochondrial enzyme that catalyzes the final step in proline biosynthesis, reducing pyrroline-5-carboxylate to L-proline with NAD(P)H as a cofactor. Related references: PMID:16730026, PMID:19648921, PMID:23024808
• At physiological concentrations, PYCR1 exhibits higher specific activity with NADH than with NADPH, linking proline metabolism to cellular redox balance. Related references: PMID:16730026, PMID:23024808
• The enzyme plays a role in the cellular response to oxidative stress, helping to maintain redox homeostasis. Related references: PMID:16730026, PMID:19648921
• PYCR1 localizes to the mitochondrion, consistent with its function in amino acid metabolism and energy production.
• Proteomics studies indicate that PYCR1 undergoes post-translational modifications such as acetylation and phosphorylation, which may regulate its activity.
• Alternative splicing of the PYCR1 gene generates multiple transcript variants, potentially expanding its functional repertoire.
• The protein is annotated with a 'Disease variant' keyword in UniProt, suggesting its involvement in human pathology.

Experimental Guidance and Technical Tips

• The immunogen used to generate this monoclonal antibody corresponds to the central region (aa 211-310) of human PYCR1, which may contribute to recognition of endogenous protein across multiple immunoassays.
• For Western blotting, a band of approximately 33 kDa is expected; consider using appropriate loading controls and lysis buffers suitable for mitochondrial proteins.
• For immunofluorescence (IF/ICC, IF-P) and immunohistochemistry (IHC-P), fixation and permeabilization conditions may need optimization to detect mitochondrial-localized PYCR1. Antigen retrieval could be beneficial for paraffin-embedded tissues.
• ELISA applications may require pairing with a suitable PYCR1 detection antibody; the clone and epitope information can guide such development.
• Validate the antibody in relevant human cell lines or tissue samples known to express PYCR1, such as those with active proline synthesis or under oxidative stress.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a streamlined sourcing platform for high-quality IVD raw materials, including the PYCR1 monoclonal antibody for proline metabolism and redox studies. Our portfolio covers validated antibody pairs, optimized recombinant antibodies, bulk ancillary reagents, and specialized raw-material procurement for rare targets. Let CamelBio be your trusted partner in advancing immunoassay development from concept to clinic.