Anti-PXK Rabbit Polyclonal Antibody for WB and ELISA - Q7Z7A4

Core Product Specifications and Parameters

Biological Background: PXK Function and Localization

• PXK (PX domain-containing protein kinase-like protein), also known as MONaKA (modulator of Na,K-ATPase), binds to and modulates brain Na,K-ATPase subunits ATP1B1 and ATP1B3, thereby participating in the regulation of electrical excitability and synaptic transmission.
• The protein likely lacks kinase activity despite its kinase-like domain classification.
• PXK localizes to the cytoplasm and cell membrane.
• It is widely expressed in tissues except the heart; isoform 1 is highly expressed in brain, skeletal muscle, spleen, and testis, while isoform 7 expression has not been demonstrated.
• The protein is annotated with keywords including actin-binding, alternative splicing, and reference proteome.
• Alternative splicing generates multiple isoforms, such as isoform 1 and isoform 7.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1-200 of human PXK, an N-terminal region likely present in multiple isoforms; validate target band size (predicted 65kDa) in your sample system.
• For Western blot, consider standard polyclonal antibody dilutions and optimize detection conditions using appropriate controls.
• The antibody is reported to cross-react with mouse and rat; validate species-specific reactivity under your experimental conditions.
• Given the cytoplasmic and cell membrane localization, efficient lysis or fractionation protocols may enhance detection.
• For ELISA, determine optimal coating antigen concentration and antibody dilution through titration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with comprehensive access to high-quality IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies like this anti-PXK antibody. For studies on Na,K-ATPase modulation and synaptic transmission, we also offer bulk ancillary reagents and can source rare targets to streamline your development pipeline from concept to clinic.