Anti-PXDN Rabbit Polyclonal Antibody for WB and ELISA - Q92626

Core Product Specifications and Parameters

Biological Background: Peroxidasin Homolog (PXDN) Function and Localization

• Peroxidasin homolog (PXDN) is a secreted heme-containing peroxidase belonging to the peroxidase family, also known as melanoma-associated antigen MG50, vascular peroxidase 1, and p53-responsive gene 2 protein.
• PXDN catalyzes the two-electron oxidation of bromide by hydrogen peroxide to generate hypobromite, which mediates the formation of sulfilimine cross-links between methionine and hydroxylysine residues in collagen IV NC1 hexamers, essential for collagen IV network assembly. Related references: PMID:18929642, PMID:19590037, PMID:22842973
• These sulfilimine cross-links are critical for the collagen IV-dependent assembly of fibronectin and laminin, and for full extracellular matrix (ECM)-mediated signaling, including growth factor-induced cell proliferation and survival in endothelial cells, thus contributing to basement membrane integrity. Related references: PMID:19590037, PMID:32543734, PMID:34679700
• PXDN may also promote tubulogenesis and angiogenesis through ERK1/2, Akt, and FAK pathways. Related references: PMID:25713063
• Additionally, PXDN brominates alpha2 collagen IV chain at Tyr-1485, leading to bromine enrichment of basement membranes. In vitro, it can oxidize thiocyanate and iodide, which can compete with bromide and inhibit sulfilimine bond formation. Related references: PMID:32571911, PMID:28154175
• PXDN is expressed in multiple tissues, with higher levels in heart, lung, ovary, spleen, intestine, and placenta, and lower levels in liver, colon, pancreas, kidney, thymus, skeletal muscle, and prostate. It is also expressed in various tumors including melanoma, breast cancer, ovarian cancer, and glioblastoma.
• Subcellularly, PXDN localizes to the endoplasmic reticulum and is secreted into the extracellular space, incorporating into the extracellular matrix and basement membrane.
• The protein contains leucine-rich repeats, immunoglobulin domains, and a peroxidase domain; it binds heme, calcium, and laminins. Disease variants and alternative splicing implicate PXDN in developmental and disease processes. Related references: PMID:32485152

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1320–1479 near the C‑terminus of human PXDN; this region may allow detection of full‑length and some processed forms. Consider validating detection of your target isoform in relevant sample systems.
• For WB, the predicted molecular weight is approximately 165 kDa; using a low‑percentage acrylamide gel and extended transfer times can improve resolution and transfer efficiency for high‑molecular‑weight proteins.
• The antibody cross‑reacts with mouse PXDN, potentially enabling studies in mouse models. However, confirm reactivity in your experimental setup.
• For ELISA, titrate the antibody against the immunogen or native protein to establish optimal working concentrations; polyclonal antibodies may require blocking optimization to minimize background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one‑stop access to IVD raw materials, including this PXDN antibody for extracellular matrix and basement membrane research. Our comprehensive portfolio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw‑material sourcing. Contact CamelBio to streamline your procurement from concept to clinic.