Anti-PXDN Rabbit Monoclonal Antibody for WB, ELISA - Q92626

Core Product Specifications and Parameters

Biological Background: PXDN Function and Localization

• PXDN (Peroxidasin homolog), also known as MG50, VPO1, and PRG2, is a secreted peroxidase belonging to the heme peroxidase family. An alternatively spliced isoform lacking the signal sequence is expressed in testis and in cells undergoing p53-dependent apoptosis, suggesting context-specific functional forms.
• Catalyzes bromide oxidation to hypobromite, which drives sulfilimine cross-link formation between methionine and hydroxylysine residues in collagen IV NC1 hexamers. This cross-linking is critical for collagen IV network assembly, fibronectin and laminin deposition, and extracellular matrix (ECM)-mediated signaling essential for basement membrane integrity.
  Related references: PMID:18929642 PMID:19590037 PMID:22842973 PMID:32543734 PMID:34679700
• Promotes endothelial cell tubulogenesis and angiogenesis via ERK1/2, Akt, and FAK signaling pathways, highlighting a role in vascular biology.
  Related references: PMID:25713063
• Brominates alpha2 collagen IV chain (COL4A2) at Tyr-1485, leading to bromine enrichment of basement membranes and potentially influencing ECM structure and function.
  Related references: PMID:32571911
• Exhibits broad substrate specificity; in vitro, it can oxidize thiocyanate and iodide, which compete with bromide and thereby inhibit sulfilimine bond formation, indicating possible regulatory mechanisms.
  Related references: PMID:28154175
• Binds laminins directly, which may facilitate its localization and function within the ECM; additionally, it may participate in eyeball and lens development during ocular morphogenesis.
  Related references: PMID:32485152
• Widely expressed in adult tissues, with highest levels in heart, lung, ovary, spleen, intestine, and placenta, and lower in liver, colon, kidney, and skeletal muscle. Also detected in several tumors including melanoma, breast, ovarian, and glioblastoma.
• Subcellularly, PXDN is found in the endoplasmic reticulum, at the cell surface, and as a secreted protein in the extracellular matrix and basement membrane, consistent with its role in ECM modification.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein covering amino acids 431-520 of human PXDN (NP_036425.1). Validate specificity in your target species and tissue context, as cross‑reactivity with endogenous PXDN may vary.
• For Western blot, the predicted molecular weight is approximately 165 kDa. Consider using a gel with appropriate acrylamide percentage and transfer conditions optimized for high‑molecular‑weight proteins.
• This antibody is suitable for ELISA; test a range of coating concentrations and sample dilutions to establish optimal signal‑to‑noise in your assay format.
• Cross‑reactivity with mouse and rat PXDN has been reported, making it a candidate for translational studies; however, always confirm reactivity in your specific model system.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutions by providing a comprehensive portfolio of IVD raw materials, including validated monoclonal antibodies, antigen‑matched pairs, and ancillary reagents. This PXDN rabbit monoclonal antibody complements our extracellular matrix and vascular biology reagent lines, enabling streamlined assay development and biomarker studies. From concept‑to‑clinic, we offer technical consultation and supply‑chain solutions to accelerate your IVD projects.