Anti-PWP1 Monoclonal Antibody for WB, ELISA - Q13610

Core Product Specifications and Parameters

Biological Background: PWP1 Function and Localization

• PWP1 (Periodic tryptophan protein 1 homolog, also known as Keratinocyte protein IEF SSP 9502) is a chromatin-associated factor that regulates transcription.
  Related references: PMID:29065309
• It positively regulates RNA polymerase I-mediated rRNA biogenesis and also modulates RNA polymerase III-mediated transcription.
  Related references: PMID:29065309
• PWP1 controls the epigenetic status of ribosomal DNA (rDNA), influencing chromatin structure at these loci.
  Related references: PMID:29065309
• The protein contains WD repeats, which are likely involved in coordinating multi-protein complex assemblies.
• Subcellularly, PWP1 localizes to the nucleus, accumulating in the nucleolus and associating with chromosomes.
• Tissue expression is broad, with highest levels in placenta, skeletal muscle, kidney, and pancreas, and lower expression in heart, brain, and lung.
• PWP1 undergoes alternative splicing and phosphorylation, as identified by proteomic studies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 460–501, a region in the C-terminal portion of PWP1. This design may favor detection of full-length protein; verify specificity in your model system.
• For Western blot, the predicted molecular weight is ~56 kDa. Optimize sample preparation and antibody dilution using a known positive lysate (e.g., HeLa, HEK293, or relevant tissue).
• In ELISA, consider using recombinant PWP1 protein as a standard; antibody performance may depend on coating and blocking conditions.
• Due to possible alternative splicing and post-translational modifications, confirm the observed band or signal against the expected size and tissue profile.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-PWP1 monoclonal antibody supports research into transcriptional regulation and ribosome biogenesis. Beyond antibodies, our supply network includes validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary materials, and sourcing of rare target raw materials to accelerate your assay development.