Anti-PVRL4 (Nectin-4) Rabbit Polyclonal Antibody for WB and ELISA - Q96NY8

Core Product Specifications and Parameters

Biological Background: Nectin-4 Function and Localization

• Nectin-4 (also known as PVRL4, LNIR, PRR4) is a member of the nectin family of immunoglobulin-like cell adhesion molecules.
• It mediates cell adhesion through trans-homophilic interactions and heterophilic interactions with NECTIN1, contributing to tissue organization.
• Acts as a cellular receptor for measles virus, facilitating viral entry into host cells.
• Predominantly expressed in placenta; not detected in normal breast epithelium but overexpressed in breast carcinoma, suggesting a role in tumor progression.
• Localizes to the cell membrane and adherens junctions; a secreted form has been identified.
• Contains three Ig-like domains in its extracellular region and is post-translationally modified by glycosylation.
• Alternative splicing generates multiple isoforms; mutations in the NECTIN4 gene are associated with ectodermal dysplasia syndromes.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 32-146) of human PVRL4, which encompasses the first Ig-like domain; the antibody may detect both native and denatured forms.
• For Western blotting, consider using placental tissue lysates or breast carcinoma cell lines (e.g., MCF-7) as positive controls, where the 55 kDa band should be apparent.
• For ELISA, the recombinant immunogen can serve as a capture standard or coating antigen; validate sensitivity and specificity in the relevant sample matrix.
• Cross-reactivity with mouse and rat indicates epitope conservation; confirm detection in these species using appropriate tissue extracts or cultured cells.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with one-stop access to IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies for targets like nectin-4. Our supply capabilities cover bulk ancillary reagents and rare-target raw-material sourcing to support your measles virus detection or cancer biomarker assay development. Let CamelBio bridge your workflow from concept to clinic.