Anti-PVRL1 Rabbit Polyclonal Antibody for WB, ELISA - Q15223

Core Product Specifications and Parameters

Biological Background: Nectin-1 Function and Localization

• Nectin-1 is a cell adhesion molecule that promotes cell-cell contacts and plays important roles in the development of the nervous system. It forms homophilic or heterophilic trans-dimers, interacting with nectin-3 and nectin-4. Related references: PMID:21325282
• Acts as a receptor for herpes simplex virus 1 (HSV-1), HSV-2, and pseudorabies virus, serving as the major entry mediator for HSV-1 into host cells. Related references: PMID:21980294, PMID:25231300, PMID:28381567, PMID:34587223
• Involved in axon guidance and synaptogenesis by promoting contacts between commissural axons and floor plate cells, and has neurite outgrowth-promoting activity.
• Promotes the formation of a checkerboard-like cellular pattern in the auditory epithelium via heterophilic interaction with nectin-3, where it is present on hair cells.
• Required for enamel mineralization.
• Localized at the cell membrane, adherens junctions, and presynaptic cell membrane; a secreted form is also produced.
• Undergoes alternative splicing and post-translational modifications including glycosylation, phosphorylation, and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal intracellular region (amino acids 450-517), which may provide good accessibility in denatured Western blot samples. Consider using whole cell lysates from tissues with known nectin-1 expression (e.g., brain, epithelial cells) for positive controls.
• For Western blot, a band of approximately 57 kDa is expected; optimize antibody dilution for your specific experimental conditions.
• For ELISA, this antibody may be used for direct detection of recombinant nectin-1 or as part of a sandwich pair after validating performance in your assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated provider of IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. With expertise in cell adhesion and viral entry targets, CamelBio can support your development of diagnostic assays by supplying high-quality anti-PVRL1 antibodies and assisting in sourcing rare target raw materials. Connect with us to streamline your raw material procurement from concept to clinic.