Anti-PUS7 Rabbit Polyclonal Antibody for WB, ELISA - Q96PZ0

Core Product Specifications and Parameters

Biological Background: PUS7 Function and Localization

• PUS7 (pseudouridylate synthase 7 homolog) catalyzes the pseudouridylation of various RNAs, including tRNAs, mRNAs, and pre-mRNAs, converting uridine to pseudouridine. Related references: PMID:28073919, PMID:29628141, PMID:30778726
• In embryonic stem cells, PUS7 regulates protein synthesis by pseudouridylating tRNA-derived fragments (tRFs), which then inhibit translation initiation. Related references: PMID:29628141
• PUS7 mediates mRNA pseudouridylation at the consensus sequence 5'-UGUAG-3', impacting mRNA stability and function. Related references: PMID:28073919, PMID:31477916, PMID:35051350
• It acts as a regulator of pre-mRNA splicing by pseudouridylating pre-mRNAs near splice sites, directly modulating alternative splicing and mRNA 3'-end processing. Related references: PMID:35051350
• PUS7 binds to a broad spectrum of RNA types, including snRNAs, Y RNAs, and vault RNAs, suggesting a wide role in RNA modification beyond tRNAs and mRNAs. Related references: PMID:29628141
• The protein localizes predominantly to the nucleus, consistent with its functions in RNA processing.
• Mutations in PUS7 are associated with human diseases such as intellectual disability and dwarfism, underscoring its biological importance.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 352–661, a central region likely to generate antibodies recognizing the full-length protein; confirm specificity in your experimental system.
• For Western blotting, the predicted molecular weight is approximately 75 kDa; consider using nuclear-enriched lysates due to PUS7 nuclear localization.
• As a rabbit polyclonal antibody, it may detect multiple isoforms or cleavage products; titrate and optimize dilutions for your sample type.
• Cross-reactivity with mouse and rat is reported, making it suitable for studies in common mammalian models; validate in your target species.
• For ELISA applications, consider coating with the recombinant immunogen or full-length protein and pairing with appropriate detection reagents.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of raw materials for IVD development, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our one-stop sourcing covers rare targets like PUS7, supporting research in RNA biology, pseudouridylation, and splicing. Whether you need research-grade antibodies or GMP-ready raw materials, we offer tailored supply solutions to bridge your concept-to-clinic pipeline.