Anti-PUS1 Rabbit Polyclonal Antibody for WB, ELISA - Q9Y606

Core Product Specifications and Parameters

Biological Background: PUS1 Function and Localization

• PUS1 is a pseudouridylate synthase that catalyzes the isomerization of uridine to pseudouridine in tRNA and mRNA substrates. Related references: PMID:15772074 PMID:24722331
• It acts on specific positions in the anticodon stem and loop of tRNAs, including positions 27/28, 34, and 36. Related references: PMID:24722331
• Also mediates pseudouridylation of mRNAs, recognizing the consensus sequence 5'-UGUAG-3'. Related references: PMID:31477916 PMID:35051350
• Functions as a regulator of pre-mRNA splicing by pseudouridylating pre-mRNAs near splice sites, impacting alternative splicing and 3'-end processing. Related references: PMID:35051350
• Involved in modulating nuclear receptor activity through pseudouridylation of the SRA1 mRNA. Related references: PMID:24722331
• Localizes to mitochondria, nucleus, and cytoplasm, reflecting its diverse roles in RNA modification.
• Widely expressed across tissues, with highest levels in brain and skeletal muscle. Related references: PMID:15108122
• Keywords include isomerase, mRNA processing, tRNA processing, alternative splicing, and primary mitochondrial disease, highlighting its multifaceted cellular roles.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 308–427) of human PUS1, which may be suitable for applications requiring detection of full-length protein.
• For Western blot, a band of approximately 47 kDa is expected in human cell or tissue lysates; consider using brain or skeletal muscle samples due to higher expression.
• For ELISA, validate the antibody performance with purified recombinant PUS1 or cell extracts from relevant human cell lines.
• The polyclonal nature may exhibit batch-to-batch variability; we recommend titration and optimization for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, offering validated antibodies, optimized reagents, and rare-target procurement for RNA modification and mitochondrial disease research. We support diagnostic manufacturers with bulk ancillary reagents and polyclonal antibodies like this anti-PUS1 pAb, ensuring streamlined supply from concept to clinic.