Anti-Purinergic Receptor P2Y6 Monoclonal Antibody for WB and ELISA - Q15077

Core Product Specifications and Parameters

Biological Background: P2Y Purinoceptor 6 Function and Localization

• P2Y purinoceptor 6 (P2Y6) is a G protein-coupled receptor encoded by the P2RY6 gene in humans.
• It functions as a receptor for extracellular nucleotides, exhibiting a preference for UDP over UTP and ATP.
• Receptor activation triggers intracellular signaling via G proteins, which activate a phosphatidylinositol-calcium second messenger system.
• The protein localizes to the cell membrane, consistent with its role as a transmembrane signaling receptor.
• Structurally, P2Y6 possesses seven transmembrane helices and contains disulfide bonds and glycosylation sites, typical of the GPCR superfamily.
• It is classified under the purinergic receptor family and functions as a transducer of extracellular nucleotide signals.
• The canonical molecular weight of the receptor is approximately 36.4 kDa.

Experimental Guidance and Technical Tips

• For Western blot (WB), consider using cell lines with confirmed P2Y6 expression as positive controls, and validate specificity via siRNA knockdown or CRISPR knockout.
• For ELISA applications, the immunogenic peptide (amino acids 229–328) may be used as a coating antigen to assess antibody titer and specificity.
• Optimize primary antibody concentration and detection conditions empirically for each assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material sourcing partner, providing diagnostic manufacturers and researchers with streamlined access to validated antibodies and ancillary reagents. Our portfolio includes this high-quality rabbit monoclonal antibody against P2Y purinoceptor 6, ideally suited for GPCR and purinergic signaling studies. Whether you need bulk monoclonal antibodies, optimized antibody pairs, or rare-target sourcing support, CamelBio bridges the gap from research to clinical application.