Anti-Pumilio 2 Monoclonal Antibody for WB, ELISA - Q8TB72

Core Product Specifications and Parameters

Biological Background: Pumilio 2 Function and Localization

• Pumilio homolog 2 (PUM2) is a sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the Pumilio Response Element (PRE) in the 3'-UTR of target mRNAs. Related references: PMID:21397187
• PUM2 mediates translational inhibition and mRNA degradation through direct recruitment of the CCR4-POP2-NOT deadenylase complex. Related references: PMID:22955276
• It also promotes miRNA-mediated repression in a deadenylation-independent manner, facilitating miRNA accessibility to target sites. Related references: PMID:18776931, PMID:22345517
• PUM2 represses E2F3 mRNAs by binding their 3'-UTR, linking it to cell cycle regulation. Related references: PMID:22345517
• The protein is involved in cytoplasmic sensing of viral infection and is regulated by the lncRNA NORAD, which sequesters PUM2 to modulate genomic stability gene programs. Related references: PMID:25340845, PMID:26724866
• PUM2 may regulate DCUN1D3 mRNA levels and support stem cell proliferation and self-renewal. Related references: PMID:25349211
• Together with PUM1, it binds the miRNA MIR199A precursor to regulate its post-transcriptional expression. Related references: PMID:28431233
• Subcellularly, PUM2 localizes predominantly to the cytoplasm, often enriched in perinuclear granules, consistent with its role in mRNA regulation.
• It is highly expressed in testis and ovary, with lower levels in brain, heart, kidney, and other tissues; at the protein level, it is detected in male germ cells of adult testis.
• Keywords associated with PUM2 include alternative splicing, methylation, phosphoprotein, and RNA-binding, indicating multiple regulatory layers and post-translational modifications.

Experimental Guidance and Technical Tips

• The immunogen peptide corresponds to the N-terminal region (aa 1–100) of human PUM2. For Western blot, consider using denaturing conditions to detect the full-length protein at ~114 kDa.
• Cross-reactivity with mouse and rat is indicated; validate detection in relevant rodent cell lines or tissues.
• In ELISA, the peptide antigen may be used as a positive control; optimize coating conditions and antibody dilution.
• For functional studies, note that PUM2 acts as a translational repressor; consider using the antibody in RNA immunoprecipitation (RIP) to assess RNA-binding activity in your system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, labs, and research institutes with one-stop sourcing of IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and rare target raw materials. For studies on RNA-binding proteins, post-transcriptional regulation, or stem cell biology, this Pumilio 2 antibody can be integrated into your workflow, with CamelBio ready to assist your procurement needs.