Anti-Pumilio 1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q14671

Core Product Specifications and Parameters

Biological Background: Pumilio 1 Function and Localization

• Pumilio homolog 1 (PUM1), also known as PUMH or HSPUM, is a ~126 kDa RNA-binding protein and a member of the Pumilio family of post-transcriptional regulators.
• PUM1 binds the Pumilio Response Element (PRE) 5'-UGUANAUA-3' in target mRNA 3'-UTRs, mediating post-transcriptional repression via deadenylation-dependent (CCR4-POP2-NOT) and deadenylation-independent (miRNA accessibility) mechanisms. Related references: PMID:18328718, PMID:21397187, PMID:21572425, PMID:22955276, PMID:18776931, PMID:20818387, PMID:20860814
• PUM1 regulates cell cycle progression by binding CDKN1B/p27 mRNA, inducing a conformational change that exposes miRNA binding sites for miR-221/222, leading to rapid cell cycle entry. Related references: PMID:20818387
• It also represses E2F3 and ATXN1 mRNAs through 3'-UTR interactions, with implications for cell proliferation and neuronal function. Related references: PMID:22345517, PMID:29474920, PMID:25768905
• PUM1 maintains genomic stability by repressing key mitotic, DNA repair, and replication factors, with activity regulated by the lncRNA NORAD. Related references: PMID:26724866
• It plays a role in cytoplasmic sensing of viral infection and post-transcriptional regulation of spermatogenesis. Related references: PMID:25340845
• PUM1 binds precursor miRNA MIR199A and, together with PUM2, regulates its expression. Related references: PMID:28431233
• Subcellularly, PUM1 localizes to the cytoplasm, P-bodies, and cytoplasmic granules. It is expressed in brain, heart, kidney, muscle, intestine, and stomach, but not in certain brain regions like cerebellum and hippocampus.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight of PUM1 is ~126 kDa; verify band specificity using appropriate controls.
• In immunocytochemistry (IF/ICC), PUM1 exhibits cytoplasmic staining, often enriched in P-bodies; co-staining with P-body markers is recommended for confirmation.
• Given the polyclonal nature, lot-to-lot consistency should be assessed; titration experiments are advised for optimal performance in each application.
• Cross-reactivity with mouse and rat PUM1 has been reported; validate detection in the relevant species and tissue types.

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CamelBio offers comprehensive sourcing solutions for IVD manufacturers, providing high-quality raw materials such as this anti-PUM1 polyclonal antibody for RNA-binding protein research. Our supply capabilities include validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents to support your diagnostic development pipeline. We help bridge the gap from concept to clinic with reliable, one-stop raw material access.