Anti-Pumilio 1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q14671

Core Product Specifications and Parameters

Biological Background: Pumilio 1 Function and Localization

• Pumilio homolog 1 (PUM1) is a sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding to the 3'-UTR of target mRNAs via the Pumilio Response Element (PRE) consensus sequence 5'-UGUANAUA-3'. Related references: PMID:18328718, PMID:21397187, PMID:21572425

• PUM1 mediates repression through two mechanisms: direct recruitment of the CCR4-POP2-NOT deadenylase complex leading to translational inhibition and mRNA degradation, and promotion of miRNA accessibility for deadenylation-independent repression. Related references: PMID:22955276, PMID:18776931, PMID:20818387, PMID:20860814

• Following growth factor stimulation, PUM1 is phosphorylated and binds to the 3'-UTR of CDKN1B/p27 mRNA, inducing a conformational change that exposes miRNA-binding sites for miR-221 and miR-222, resulting in efficient suppression of CDKN1B/p27 and rapid cell cycle entry. Related references: PMID:20818387

• PUM1 represses a program of genes essential for genomic stability, including mitotic, DNA repair, and DNA replication factors; this activity is antagonized by the lncRNA NORAD, which sequesters PUM1 in the cytoplasm. Related references: PMID:26724866

• In neurons, PUM1 regulates ATXN1 mRNA levels by binding to its 3'-UTR and down-regulating transcripts independently of the miRNA machinery, with implications for spinocerebellar ataxia. Related references: PMID:25768905, PMID:29474920

• PUM1 localizes to the cytoplasm, P-bodies, and cytoplasmic granules, and is broadly expressed in tissues including brain, heart, kidney, muscle, intestine, and stomach, but is absent from specific brain regions such as cerebellum, corpus callosum, and hippocampus.

• PUM1 plays roles in cytoplasmic viral sensing, spermatogenesis, and embryonic stem cell renewal by targeting mRNAs for post-transcriptional regulation, and is associated with disease variants linked to epilepsy, intellectual disability, and neurodegeneration. Related references: PMID:25340845

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-130 of human Pumilio 1, targeting the N-terminal region. This region may be conserved across species, supporting the observed cross-reactivity with mouse.
• For Western blotting, the predicted molecular weight is ~126 kDa. Use appropriate gel percentage and transfer conditions for high-molecular-weight proteins, and consider extended blocking and washing steps to reduce background.
• In immunofluorescence/ICC, PUM1 cytoplasmic and granular localization should be assessed with appropriate fixation and permeabilization; validate the staining pattern against known P-body or stress granule markers if needed.
• For ELISA applications, the recombinant protein immunogen can serve as a positive control. Titrate the antibody to optimize signal-to-noise ratio.
• As a polyclonal antibody, lot-to-lot variability may occur; we recommend validating each new lot in your specific assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials, technical services, and consulting support from concept to clinic. For Pumilio 1 and RNA-binding protein studies, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to streamline your assay development. Contact us to explore how our one-stop supply chain can accelerate your project.