Anti-PTRH1 Rabbit Polyclonal Antibody for WB, ELISA - Q86Y79

Core Product Specifications and Parameters

Biological Background: PTRH1 Function and Localization

• PTRH1 (Peptidyl-tRNA hydrolase), also known as PTH1 or C9orf115, is a 23 kDa hydrolase that binds tRNA and RNA.
• It functions in the ribosome-associated quality control (RQC) pathway, cleaving nascent polypeptide chains from tRNA when they are not properly positioned in the P-site of the 60S ribosomal subunit. Related references: PMID:30244831
• PTRH1 specifically targets non-ubiquitinated substrates; ubiquitinated nascent chains are processed separately by ANKZF1. Related references: PMID:30244831
• The enzyme is part of the reference proteome and is involved in maintaining translational fidelity.
• Its UniProt annotation includes the keywords hydrolase, RNA-binding, and tRNA-binding, reflecting its catalytic and nucleic acid interaction properties.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 1–214 of human PTRH1, covering nearly the full-length protein, which may generate antibodies recognizing multiple epitopes.
• For western blotting, consider using standard reducing SDS-PAGE; a band of approximately 23 kDa is expected in human cell lysates.
• For ELISA, consider testing against the immunogen or recombinant PTRH1 protein as a positive control.
• Validate specificity in the relevant sample system; cross-reactivity with other species has not been determined.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials, technical services, and consulting from concept to clinic. This anti-PTRH1 antibody supports research in translational quality control and proteostasis. We provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your development and manufacturing workflows.