Anti-PTRF Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q6NZI2

Core Product Specifications and Parameters

Biological Background: PTRF Function and Localization

• PTRF (Polymerase I and transcript release factor), also designated Cavin-1, CAVIN, FKSG13, or CGL4, is a caveolae-associated protein encoded by the CAVIN1 gene in humans.
• Plays an essential role in caveolae formation and organization in all tissues. As a core component of the CAVIN complex, it is required for caveolae biogenesis in the presence of caveolin-1 (CAV1) and for proper CAV1 oligomerization. Related references: PMID:18056712; PMID:18191225; PMID:19726876
• Regulates ribosomal transcription: enhances ribosomal transcriptional activity upon metabolic stimulation in adipocytes, contributes to ribosomal transcriptional loop formation, and releases paused RNA polymerase I and pre-rRNA from TTF1-bound DNA templates. Related references: PMID:18056712; PMID:18191225; PMID:19726876
• Subcellular localization includes membrane, caveola, and cell membrane, as well as microsome, endoplasmic reticulum, cytoplasm, cytosol, mitochondrion, and nucleus.
• Subject to multiple post-translational modifications: phosphorylation, acetylation, isopeptide bond formation, and ubiquitination.
• Genetic variations in CAVIN1 are linked to congenital generalized lipodystrophy and diabetes mellitus, underscoring its importance in metabolic homeostasis.

Experimental Guidance and Technical Tips

• The immunogen (residues 200–280 of human PTRF) targets a conserved central region, enabling potential cross-reactivity with mouse and rat orthologs.
• For Western blot, anticipate a main band near 43 kDa; additional bands may arise from post-translational modifications or isoform diversity—validate using positive controls.
• IHC-P and IF/ICC work best in caveolae-rich tissues (e.g., adipose, muscle, endothelium); consider nuclear/cytoplasmic staining patterns as the protein shuttles between compartments.
• For ELISA applications, optimise antibody concentration and coating conditions using recombinant protein or cell lysates to assess linearity and specificity.
• As a polyclonal preparation, lot-to-lot consistency should be verified, and antibody titration is recommended for each new assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This PTRF antibody supports research into caveolae biology, adipocyte function, and metabolic disorders. Our portfolio includes validated polyclonal and monoclonal antibodies, matched antibody pairs, and ancillary reagents, along with the capability to source rare-target raw materials to streamline your IVD development and production.