Anti-PTPRR Rabbit Polyclonal Antibody for WB, ELISA - Q15256

Core Product Specifications and Parameters

Biological Background: PTPRR Function and Localization

• PTPRR (Receptor-type tyrosine-protein phosphatase R) is a member of the protein tyrosine phosphatase (PTP) family, also known by aliases including PTPRQ, EC-PTP, PCPTP1, PTP-SL, and PTPBR7.
• Its primary function is to sequester mitogen-activated protein kinases (MAPKs) such as MAPK1, MAPK3, and MAPK14 in the cytoplasm, maintaining them in an inactive form; phosphorylation of the kinase interaction motif by PKA promotes MAPK release and nuclear translocation (By similarity).
• The protein exhibits multiple subcellular localizations: it is found at the cell membrane, in the perinuclear cytoplasmic region, and is also secreted.
• Post-translational modifications include phosphorylation and glycosylation; it contains an N-terminal signal peptide and a transmembrane domain, consistent with a receptor-type phosphatase topology (Keywords: Glycoprotein, Phosphoprotein, Signal, Transmembrane).
• Tissue expression is broad: detected in brain, placenta, small intestine, stomach, uterus, and weakly in prostate; notably, the protein is present in cerebrospinal fluid (PubMed:25326458). Isoform alpha is restricted to brain, while gamma and delta isoforms are also found in placenta and uterus (gamma) or kidney and prostate (delta). Related references: PMID:25326458

Experimental Guidance and Technical Tips

• For Western blot, the predicted molecular weight is 74 kDa; however, alternative splicing may produce isoforms of different sizes. Validate using appropriate positive controls and consider isoform-specific references.
• The immunogen (aa22–227) lies within the N-terminal region shared by multiple isoforms; confirm specificity by knockdown or isoform-specific antibodies where necessary.
• Validated for ELISA; when developing a sandwich immunoassay, screen the antibody in combination with a suitable detection antibody for human and rat samples.
• High sequence homology between human and rat PTPRR supports observed cross-reactivity; always validate performance in the target species under your experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio serves as your comprehensive partner for IVD raw-material supply, providing validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for specialized targets like PTPRR.
• Focusing on the MAPK signaling and phosphatase research area, we integrate reliable procurement with technical consultation to accelerate your diagnostic development from early-stage assay design to clinical readiness.