Anti-PTPRN2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q92932

Core Product Specifications and Parameters

Biological Background: PTPRN2 Function and Localization

• PTPRN2 (Receptor-type tyrosine-protein phosphatase N2), also known as IA-2beta, phogrin, IAR, and ICAAR, is a type I transmembrane protein belonging to the protein-tyrosine phosphatase family.
• It plays a crucial role in vesicle-mediated secretory processes, being required for normal accumulation of secretory vesicles in the hippocampus, pituitary, and pancreatic islets (By similarity).
• Required for the accumulation of normal levels of insulin-containing vesicles and preventing their degradation, thus playing a role in insulin secretion in response to glucose stimuli (By similarity).
• Involved in neurotransmitter accumulation in the brain, required for normal levels of norepinephrine, dopamine, and serotonin (By similarity).
• Exhibits phosphatidylinositol phosphatase activity, dephosphorylating PI(4,5)P2, which regulates actin dynamics and cell migration (PubMed:26620550). Related references: PMID:26620550
• Subcellularly localized to the membrane of secretory vesicles, including synaptic vesicles and secretory granule membranes.
• Highest expression in brain and pancreas, with lower levels in trachea, prostate, stomach, and spinal cord (PubMed:8798755, PubMed:8954911). Related references: PMID:8798755, PMID:8954911
• Key post-translational modifications include glycosylation, acetylation, and phosphorylation; alternative splicing produces multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular region (aa 22–242), likely exposed in both denaturing and native conditions, making the antibody suitable for WB, IHC-P, and ELISA.
• For Western blot, the full-length protein migrates at ~111 kDa; ensure adequate gel separation and transfer conditions.
• For IHC-P, antigen retrieval may be considered and validation in positive control tissues such as pancreas or brain is recommended.
• Cross-reactivity with mouse and rat enables comparative studies across species; however, verify specificity in the relevant experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials and tailored sourcing services to support diagnostic manufacturers, labs, and research institutes from concept to clinic. This anti-PTPRN2 polyclonal antibody is part of our extensive catalog for metabolic and neuroscience research applications. Our team can assist with validated antibody pairs, recombinant proteins, bulk ancillary reagents, and custom rare-target material procurement to accelerate your project.