Anti-PTPRF Rabbit Polyclonal Antibody for WB and ELISA - P10586

Core Product Specifications and Parameters

Biological Background: PTPRF Function and Localization

• PTPRF, also known as Leukocyte common antigen related (LAR), is a receptor-type tyrosine-protein phosphatase that contains extracellular immunoglobulin-like and fibronectin type III domains.
• It functions as a cell adhesion receptor and possesses intrinsic protein tyrosine phosphatase (PTPase) activity, notably dephosphorylating EPHA2 to regulate its activity.
• The protein has two tandem PTPase domains: the first domain (D1) carries the catalytic activity, while the second domain (D2) modulates the substrate specificity of D1.
• PTPRF is a single-pass type I membrane protein localized at the plasma membrane.
• Post-translational modifications include glycosylation and phosphorylation, which may regulate its activity and interactions.
• Additional features include heparin binding, disulfide bond formation, and multiple alternative splicing variants, contributing to functional diversity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the cytoplasmic region of human PTPRF (amino acids 1016–1250), which spans part of the PTPase domains; this design supports detection of the full-length receptor.
• For Western blotting (WB), the predicted molecular weight is approximately 213 kDa; consider using a low-percentage polyacrylamide gel (e.g., 6% gel) for optimal resolution of high-molecular-weight proteins.
• Validate antibody specificity in your experimental system using appropriate positive (e.g., cell lines expressing PTPRF) and negative controls; human and mouse samples are expected to react.
• For ELISA applications, polyclonal antibodies can serve as either capture or detection reagents; cross‑titration is recommended to establish optimal working concentrations.
• As a polyclonal preparation, lot‑to‑lot variability may occur; it is advisable to titrate and confirm performance upon receipt of each new batch.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one‑stop access to a wide range of IVD raw materials, including this high‑quality rabbit polyclonal antibody targeting PTPRF/LAR for cell adhesion and phosphatase studies. Beyond individual antibodies, we supply validated antibody pairs, bulk monoclonal and polyclonal antibodies, ancillary reagents, and specialized raw material sourcing for rare or emerging targets. Contact us to streamline your procurement and accelerate your project from concept to clinic.