Anti-PTPN4 (PTPMEG) Rabbit Polyclonal Antibody for WB, ELISA - P29074

Core Product Specifications and Parameters

Biological Background: PTPN4 Function and Localization

• PTPN4 is a non-receptor protein tyrosine phosphatase involved in immune regulation, synaptic plasticity, and neuronal homeostasis.
  Related references: PMID:25825441, PMID:27246854
• It protects neurons against apoptosis, contributing to neuronal cell homeostasis.
  Related references: PMID:20086240
• PTPN4 negatively regulates TLR4-induced interferon-β production by dephosphorylating the adaptor TICAM2 (TRAM), thereby disrupting the TRAM-TRIF interaction.
  Related references: PMID:25825441
• The phosphatase also dephosphorylates ITAM motifs on the TCR ζ chain, attenuating T-cell receptor signaling (By similarity).
• PTPN4 localizes to the cell membrane, cytoplasm, and cytoskeleton, suggesting a role at membrane-cytoskeleton junctions.
• The protein belongs to the non-receptor type 4 tyrosine phosphatase family and is annotated with keywords including hydrolase, phosphoprotein, and reference proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-240) of human PTPN4; consider this when designing experiments targeting different domains.
• For Western blot, validate antibody specificity using lysates from human, mouse, and rat cells/tissues, given the reported cross-reactivity.
• For ELISA, include recombinant antigen controls to confirm binding activity.
• PTPN4 has a molecular weight of approximately 106 kDa; use appropriate gel percentage (e.g., 8–10% SDS-PAGE) for optimal separation.
• Since PTPN4 localizes to multiple subcellular compartments, consider fractionation or co-localization studies to confirm target localization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibodies, antigens, and ancillary reagents to support your assay development. For PTPN4 and phosphatase signaling research, we provide anti-PTPN4 antibodies and related products, helping you streamline procurement from concept to clinic. Contact us to explore bulk purchasing options and technical support.