Anti-PTPN3 Polyclonal Antibody for WB and ELISA - P26045

Core Product Specifications and Parameters

Biological Background: PTPN3 Function and Localization

• PTPN3 (Tyrosine-protein phosphatase non-receptor type 3) is also known as PTP-H1.
• Core function: Acts at membrane-cytoskeleton junctions and exhibits tyrosine phosphatase activity.
• Belongs to the non-receptor class of protein-tyrosine phosphatases.
• Subcellular localization includes cell membrane, cytoplasm, and association with the cytoskeleton.
• Classified as a phosphoprotein, indicating regulation by phosphorylation.
• Multiple isoforms produced by alternative splicing.
• A 3D structure has been resolved, aiding in functional studies.
• Part of the human reference proteome, validated by proteomics identification.

Experimental Guidance and Technical Tips

• The immunogen targets amino acids 1–300 at the N-terminal region of human PTPN3, covering a functionally relevant domain.
• For Western blotting, the expected band is at approximately 104 kDa; validate in human, mouse, or rat cell/tissue lysates known to express PTPN3.
• ELISA applications may require optimization of antibody coating concentration and pairing with appropriate detection reagents.
• Cross-reactivity with rodent species broadens utility in translational models; confirm reactivity in your specific system.
• Use standard controls (e.g., positive cell lines, peptide blocking) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw materials and technical support, from concept to clinic. For your phosphatase signaling research, we provide validated anti-PTPN3 antibodies, customized antibody pairs, and bulk ancillary reagents. Contact our team to source rare targets or optimize your immunoassay workflow with our expert guidance.