Anti-PTPN1 Polyclonal Antibody for WB, ELISA - P18031

Core Product Specifications and Parameters

Biological Background: PTPN1 Function and Localization

• Encoded by the PTPN1 gene, Tyrosine-protein phosphatase non-receptor type 1 (also known as protein-tyrosine phosphatase 1B or PTP-1B) is a key regulatory phosphatase.
• Functions as a tyrosine-protein phosphatase and regulates the endoplasmic reticulum unfolded protein response by dephosphorylating and inactivating EIF2AK3/PERK.
• May participate in CKII- and p60c-src-induced signal transduction cascades, and modulate the EFNA5-EPHA3 signaling pathway affecting cell reorganization and repulsion.
• Potentially regulates hepatocyte growth factor receptor signaling through dephosphorylation of the MET receptor.
• Localizes to the endoplasmic reticulum membrane, anchoring its catalytic domain in the cytoplasm.
• Expressed in keratinocytes at the protein level, suggesting roles in epithelial cell signaling.
• Classified under multiple keywords including hydrolase, protein phosphatase, and phosphoprotein; undergoes post-translational modifications such as acetylation, oxidation, and S-nitrosylation.
• Its molecular weight is approximately 50kDa (calculated 49967 Da), consistent with the detected band in WB.

Experimental Guidance and Technical Tips

• The immunogen used spans amino acids 281-435 of human PTPN1, a region within the C-terminal catalytic domain; this antigen design may contribute to high specificity for the target protein.
• For Western blot, a band around 50kDa is expected; confirm molecular weight in the relevant sample system and consider appropriate blocking and antibody dilution conditions.
• The antibody is validated for ELISA; optimize coating antigen and detection conditions for your specific assay format.
• Cross-reactivity has been observed with human and rat samples; reactivity with other species should be empirically determined.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio supports diagnostic developers and researchers by consolidating sourcing of performance-driven antibodies, including anti-PTPN1 polyclonal antibodies for phosphatase signaling studies. We offer a streamlined supply of validated antibody pairs, monoclonal/polyclonal reagents, bulk ancillary materials, and custom sourcing for rare targets—designed to accelerate your assay development from concept to clinic. Reach out to explore how we can tailor our capabilities to your project’s unique requirements.