Anti-PTP4A3 Polyclonal Antibody for WB, IHC-P, ELISA - O75365

Core Product Specifications and Parameters

Biological Background: PTP4A3 Function and Localization

• PTP4A3 (also known as PRL-3 or PRL-R) is a member of the protein tyrosine phosphatase type IVA family, which stimulates progression from G1 into S phase during mitosis and enhances cell proliferation, motility, and invasive activity.
• This phosphatase plays a critical role in promoting cancer metastasis, and its overexpression is consistently observed in colon cancer metastases.
• In cardiac tissue, PTP4A3 may contribute to the progression of cardiac hypertrophy by inhibiting intracellular calcium mobilization in response to angiotensin II.
• The protein localizes to the cell membrane and early endosomes, consistent with its involvement in signal transduction and membrane trafficking.
• PTP4A3 undergoes prenylation, a post-translational modification that facilitates membrane association, and is also predicted to have a disulfide bond and methylation sites.
• Tissue expression is highest in cardiomyocytes and skeletal muscle, with additional presence in the pancreas.
• Alternative splicing produces multiple isoforms, contributing to functional diversity in different cellular contexts.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length protein (amino acids 1-148), which may provide strong reactivity in applications such as WB, IHC-P, and ELISA. For WB, an expected band around 20 kDa should be detected.
• For IHC-P, consider validating the antibody in tissues with known PTP4A3 expression, such as cardiomyocytes, skeletal muscle, or colon cancer metastases.
• Cross-reactivity with mouse allows for use in mouse xenograft or transgenic models; validate in the relevant sample system to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories streamlined access to high-quality IVD raw materials, including this rabbit polyclonal antibody against the cancer metastasis target PTP4A3. Beyond individual antibodies, we can source validated antibody pairs, optimized monoclonal/polyclonal options, and bulk ancillary reagents to support your assay development. Whether you need rare-target raw materials or comprehensive technical consulting, CamelBio serves as a single bridge from concept to clinic.