Anti-PTP4A2 Polyclonal Antibody for WB, IHC-P, IF/ICC - Q12974

Core Product Specifications and Parameters

Biological Background: PTP4A2 Function and Localization

• PTP4A2 is a protein tyrosine phosphatase belonging to the PRL family, also designated PRL-2, and plays a role in cell cycle progression by promoting the G1-to-S phase transition.
• It exhibits oncogenic activity and is reported to promote tumorigenesis when overexpressed.
• Additionally, PTP4A2 inhibits geranylgeranyl transferase type II (GGTase-II) by blocking the interaction between RABGGTA and RABGGTB, implicating it in the regulation of protein prenylation pathways.
• Subcellularly, PTP4A2 localizes to the cell membrane, early endosomes, and cytoplasm, with membrane association dependent on post-translational prenylation and methylation.
• It is ubiquitously expressed in human tissues, with the highest levels detected in skeletal muscle, heart, and thymus, and is notably overexpressed in prostate tumor tissue.
• The protein contains a prenylation motif and is subject to post-translational modifications including methylation and disulfide bond formation, as indicated by UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 30–100 of human PTP4A2; verify sequence homology if studying non-human species or specific isoforms to ensure expected reactivity.
• As a polyclonal antibody, lot-to-lot consistency may vary; it is advisable to validate each new batch in the intended application and sample type.
• For western blotting, the predicted molecular weight is approximately 19 kDa, but post-translational modifications such as prenylation may cause slight band shifts; include appropriate controls.
• For immunohistochemistry (IHC-P), consider optimizing antigen retrieval conditions (e.g., citrate buffer pH 6.0 or Tris-EDTA pH 9.0) based on the tissue and fixation method.
• In immunofluorescence (IF/ICC), due to its association with the cell membrane and endosomes, permeabilization with detergents like Triton X-100 may be necessary; evaluate the fixation protocol (e.g., PFA versus methanol) for optimal signal.
• When using ELISA, titrate the antibody to determine the optimal working concentration for your specific assay setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop source of IVD raw materials, supplying validated antibody pairs, monoclonal and polyclonal antibodies, and ancillary reagents for diagnostic development. For targets like PTP4A2, which is implicated in tumor biology and cell cycle regulation, CamelBio offers bulk-ready materials and customized sourcing to support assay manufacturing and biomarker research. Whether you need an existing catalog antibody or a tailored solution for rare targets, our technical team and supply network can accelerate your diagnostic pipeline.