Anti-PTP1B Monoclonal Antibody for WB, IHC-P, ELISA - P18031

Core Product Specifications and Parameters

Biological Background: PTP1B Function and Localization

• PTP1B (Tyrosine-protein phosphatase non-receptor type 1) is also known as protein-tyrosine phosphatase 1B (PTP-1B). It is a non-receptor type protein tyrosine phosphatase.
• Acts as a key regulator of the endoplasmic reticulum (ER) unfolded protein response by mediating dephosphorylation and inactivation of the kinase EIF2AK3/PERK.
• Plays a role in signal transduction cascades induced by CKII and p60c-src, and may regulate the EFNA5-EPHA3 signaling pathway involved in cell reorganization and repulsion.
• Modulates the hepatocyte growth factor receptor (MET) signaling pathway through dephosphorylation, thereby influencing downstream cellular responses.
• Located at the endoplasmic reticulum membrane, consistent with its role in ER stress signaling and protein processing.
• Expressed in keratinocytes at the protein level, suggesting a role in skin biology.
• Post-translational modifications include acetylation, oxidation, phosphorylation, and S-nitrosylation, which may regulate its activity and localization.
• Classified under hydrolase, protein phosphatase, and reference proteome; it is a phosphoprotein involved in reversible tyrosine dephosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal catalytic domain (aa 281-435), which may allow recognition of the active phosphatase region; consider this when designing inhibition or activity assays.
• For Western blotting, the predicted molecular weight is approximately 50 kDa; use appropriate gel percentages and validate detection in your sample of interest.
• In IHC-P, optimize antigen retrieval and antibody dilution for tissue-specific detection, especially in samples with expected expression like keratinocytes.
• For ELISA, pair this antibody with a suitable capture/detection system, and consider cross-reactivity with mouse PTP1B if studying orthologous systems.
• Validate the antibody’s performance in your specific experimental context, as applications listed are based on supplier data.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for diagnostic manufacturers, labs, and research institutes by offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For studies on tyrosine phosphatase signaling, metabolic disorders, or cancer, CamelBio provides this PTP1B rabbit monoclonal antibody with verified cross-reactivity to human and mouse targets. As your sourcing bridge from concept to clinic, we also assist with rare target raw-material sourcing to accelerate your assay development.