Anti-PTK6 Polyclonal Antibody for WB, IF/ICC, ELISA - Q13882

Core Product Specifications and Parameters

Biological Background: PTK6 Function and Localization

• PTK6 (Protein-tyrosine kinase 6), also known as breast tumor kinase (BRK), is a 52 kDa non-receptor tyrosine-protein kinase encoded by the PTK6 gene (Gene ID: 5753).
• It plays a context-dependent role in regulating epithelial cell differentiation and maintenance, promoting apoptosis in normal tissues while enhancing proliferation, survival, and migration in tumorigenic settings.
• Upon EGF stimulation, PTK6 phosphorylates ARAP1, which delays EGFR downregulation and sustains signaling (PubMed:20554524). Related references: PMID:20554524
• PTK6 interacts with EGFR family members (EGFR, ERBB2, ERBB3) and activates downstream pathways including AKT, PI3K, and STAT3/STAT5B to drive oncogenic signaling.
• Subcellular localization includes the cytoplasm, nucleus, cell membrane, and ruffles, with nuclear PTK6 linked to growth regulation in normal epithelia and cytoplasmic PTK6 to oncogenic activation.
• It is predominantly expressed in epithelial tissues, with highest levels in colon, and overexpressed in many cancers including breast, ovarian, colon, and prostate carcinomas; not found in heart, brain, liver, or muscle.
• Post-translational modifications: classified as a phosphoprotein (UniProt keywords), and regulated by intramolecular interactions and phosphorylation events that control its kinase activity.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 222–451, which includes part of the kinase domain; antibody may recognize both phosphorylated and unphosphorylated forms, but epitope masking by post-translational modifications should be considered in certain assay conditions.
• For Western blot, a 52 kDa band is expected; use positive controls such as human colon carcinoma (e.g., HT-29) or prostate cancer (e.g., LNCaP) cell lysates. Validate specificity with siRNA-mediated knockdown or knockout cell lines.
• In immunofluorescence, PTK6 can localize to both cytoplasmic and nuclear compartments; permeabilization with 0.1–0.5% Triton X-100 and careful fixation are recommended to avoid artifacts.
• For ELISA, the recombinant immunogen fragment can serve as a positive coating antigen; include appropriate negative controls to assess background.
• Cross-reactivity with mouse and rat is predicted based on sequence homology; however, verify in the target species using relevant tissue or cell lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your reliable one-stop partner for IVD raw materials, providing comprehensive sourcing solutions that bridge concept to clinic. For PTK6 and epithelial/cancer research, we offer validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. Whether you need rare target raw materials or support for large-scale diagnostic manufacturing, our consulting and supply capabilities help streamline your development pipeline.