Anti-PTK2B Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q14289

Core Product Specifications and Parameters

Biological Background: PTK2B Function and Localization

• Non-receptor tyrosine kinase that regulates reorganization of the actin cytoskeleton, cell polarization, migration, adhesion, spreading and bone remodeling.
• Plays a role in the regulation of the humoral immune response, required for normal marginal B-cell levels in spleen and migration of splenic B-cells, and contributes to macrophage polarization and T-cell responses.
• Functions in signaling downstream of integrin and collagen receptors, immune receptors, G protein-coupled receptors (GPCRs), and cytokine/chemokine/growth factor receptors, mediating responses to cellular stress.
• Forms multisubunit signaling complexes with SRC and SRC family kinases, leading to phosphorylation of numerous substrates and activation of pathways including PI3K/AKT1, NOS3, and MAPK cascades (MAPK1/ERK2, MAPK3/ERK1, MAPK8/JNK1).
• Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, regulating P53 activity, ubiquitination and proteasomal degradation; also phosphorylates NMDA receptors and potassium channel subunits.
• Localizes to cytoplasm, perinuclear region, cell membrane, focal adhesions, lamellipodium, cell cortex and nucleus.
• Most abundant in the brain, with highest levels in amygdala and hippocampus; also expressed in spleen, lymphocytes, and at lower levels in kidney.
• Alternative splicing produces multiple isoforms; classified as a phosphoprotein and transferase.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C‑terminal region (aa 780–1009), suggesting recognition of a region distal to the kinase domain; consider this when mapping interaction sites or using truncated constructs.
• For Western blot, expect a band at ~116 kDa; validate in lysates from brain, spleen, or Jurkat T‑cells, where PTK2B is endogenously expressed.
• For IF/ICC, PTK2B often localizes to perinuclear regions and focal adhesions; fixation and permeabilization should be optimized for your cell type to preserve these structures.
• In ELISA, the antibody can be used for direct detection or as a capture/detection pair if matched with another clone; titrate coating antigen concentration and antibody dilution for optimal signal.
• When using samples from mouse or rat, cross‑reactivity is expected but should be verified by Western blot or IF on the relevant tissue, as epitope conservation may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, research laboratories, and institutes by offering a reliable supply of validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized raw‑material sourcing for challenging targets. This PTK2B antibody complements our portfolio for focal adhesion and immune signaling research, helping you move from concept to clinic with consistent, high‑quality reagents.