Anti-PTHLH Rabbit Polyclonal Antibody for WB, ELISA - P12272

Core Product Specifications and Parameters

Biological Background: PTHLH Function and Localization

• PTHLH (Parathyroid hormone-related protein), also known as Parathyroid hormone-like protein (PLP), is a neuroendocrine peptide critical for cellular growth, development, migration, differentiation, survival, and epithelial calcium ion transport. Related references: PMID:12538599, PMID:35932760, PMID:3616618
• It exerts its effects by binding to the PTH1R receptor and activating G protein-coupled receptor signaling. Related references: PMID:19674967, PMID:35932760
• PTHLH is essential for skeletal homeostasis and regulates endochondral bone development. Related references: PMID:12538599
• During mammary gland development, PTHLH promotes mesenchymal differentiation and bud outgrowth by modulating cellular responsiveness to BMPs. Related references: PMID:12538599
• PTHLH is a potent inhibitor of osteoclastic bone resorption.
• In colon cancer, PTHLH promotes cell migration and invasion through an integrin α6/β1–Rac1 dependent mechanism. Related references: PMID:20637541
• The protein localizes to the cytoplasm and nucleus and is also secreted.
• PTHLH is ubiquitously expressed, with notable expression in the mammary gland.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 25–175, covering a large portion of the mature protein; this may provide broad epitope coverage for recognizing multiple isoforms.
• For Western blot, a band at approximately 20 kDa is expected; validate with a positive control such as cancer cell lines or mammary tissue.
• Cross-reactivity with mouse and rat is indicated; verify species-specific binding in your experimental system.
• For ELISA, consider optimizing the coating concentration and detection system for your specific application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated B2B platform offering comprehensive IVD raw material sourcing, from high-quality antibodies to ancillary reagents, for diagnostic manufacturers and research institutes. This anti-PTHLH polyclonal antibody exemplifies our portfolio supporting cancer and developmental biology research. Explore our validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk reagent options to accelerate your assay development.