Anti-PTHLH Polyclonal Antibody for WB, IHC-P, ELISA - P12272

Core Product Specifications and Parameters

Biological Background: PTHLH Function and Localization

• PTHLH is a neuroendocrine peptide critical for cellular and organ growth, development, migration, differentiation, survival, and epithelial calcium ion transport.
  Related references: PMID:12538599 PMID:35932760 PMID:3616618
• It exerts its effects by binding to PTH1R, activating G protein-coupled receptor signaling.
  Related references: PMID:19674967 PMID:35932760
• PTHLH regulates endochondral bone development and epithelial-mesenchymal interactions during mammary gland and tooth formation (By similarity).
• Required for skeletal homeostasis.
  Related references: PMID:12538599
• Promotes mammary mesenchyme differentiation and bud outgrowth by modulating mesenchymal cell responsiveness to BMPs.
  Related references: PMID:12538599
• Promotes colon cancer cell migration and invasion via integrin α6/β1-dependent activation of Rac1.
  Related references: PMID:20637541
• PTHLH is secreted but also localizes to the cytoplasm and nucleus; it is ubiquitously expressed with notable presence in the mammary gland.
• According to UniProt keywords, the protein undergoes alternative splicing, cleavage on basic residues, and is associated with disease variants.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 25–175 of human PTHLH, encompassing the mature peptide region, which supports detection of both full-length and processed forms.
• For Western blotting (WB), the predicted molecular weight is ~20 kDa; consider using positive control lysates from tissues with known PTHLH expression, such as mammary gland or bone.
• For immunohistochemistry (IHC-P), antigen retrieval optimization is recommended, and staining should be validated on paraffin-embedded sections of relevant tissues.
• For ELISA applications, the antibody may be used as a capture or detection reagent after verification in the appropriate sample matrix.
• Cross-reactivity with mouse and rat has been noted; however, endogenous protein levels may vary, necessitating species-specific validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing bridge for IVD raw materials, providing diagnostic manufacturers and researchers with access to validated antibodies like this anti-PTHLH product for studies of bone biology and oncology. Our catalog includes antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents, with dedicated support for rare target sourcing.