Anti-PTH1R Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q03431

Core Product Specifications and Parameters

Biological Background: PTH1R Function and Localization

• PTH1R (parathyroid hormone 1 receptor), also known as PTH/PTHrP type I receptor or PTH1 receptor, is encoded by the PTH1R gene and belongs to the class B G protein-coupled receptor family.
• It functions as a receptor for parathyroid hormone (PTH) and parathyroid hormone-related peptide (PTHLH), playing critical roles in calcium homeostasis and bone remodeling. Related references: PMID:10913300 PMID:18375760 PMID:19674967
• Upon ligand binding, PTH1R couples to G(s) alpha proteins to activate adenylate cyclase, leading to increased intracellular cAMP levels. Related references: PMID:20172855 PMID:30975883 PMID:35932760
• PTHLH dissociates from the receptor more rapidly than PTH, resulting in a faster decay of the cAMP response, which may have implications for differential signaling dynamics. Related references: PMID:35932760
• PTH1R is localized primarily at the cell membrane as a multi-pass transmembrane protein.
• It is expressed in most tissues, with highest abundance in kidney, bone, and liver, consistent with its role in mineral ion homeostasis.
• Post-translational modifications include glycosylation, phosphorylation, and disulfide bond formation, which may affect receptor stability and trafficking.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular domain (aa 27–188), which is exposed on the cell surface; the antibody may recognize both native and denatured forms under appropriate conditions.
• For Western blotting, a band around 66 kDa is expected, but glycosylation may cause an upward shift; reducing conditions are typically recommended.
• For immunohistochemistry (IHC-P), antigen retrieval and antibody titration are critical; frozen sections may also be tested for optimal signal.
• In ELISA, the antibody can be used for direct or indirect detection; pairing with a detection antibody may require empirical optimization.
• Cross-reactivity with mouse and rat has been reported, but sequence homology in the immunogen region should be verified for your specific experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, bulk ancillary reagents, and expert technical support for diagnostic development. Our supply capabilities extend to rare targets like PTH1R, facilitating receptor signaling research and assay construction. With a commitment to quality and reliability, we streamline your path from concept to clinic.